Liquid chromatography electrospray ionization tandem mass spectrometry（LC/ESI-MS/MS） method for quantitative estimation of moxifloxacin in human plasma

A rapid and sensitive liquid chromatography tandem mass spectrometry（LC-MS/MS） method has been developed and validated for the determination of moxifloxacin（MOXI） in human plasma. After a simple protein precipitation using acetonitrile, the post treatment samples were analysed on a C18 column interfaced with a Triple Quadropole Tandem Mass Spectrometer. Positive electrospray ionization was employed as the ionization source. The mobile phase consisted of 0.1% formic acid–acetonitrile（60：40, v/v）. Ciprofloxacin（CIPRO） was used as an internal standard. The analyte and internal standard（CIPRO） were monitored in the multiple reaction monitoring mode（MRM）. The mass transition ion-pair has been followed as m/z 402→358.2 for MOXI and 332→288.1 for CIPRO. The method was linear in the concentration range of 25–5000 ng/mL. The lower limit of quantification was 25 ng/mL. The intra- and inter-day precision（relative standard deviation） and accuracy（relative error） values were within 12.4%. Each plasma sample was analyzed within 3 min.

Liquid chromatography electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) method for quantitative estimation of moxifloxacin in human plasma

A rapid and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the determination of moxifloxacin (MOXI) in human plasma. After a simple protein precipitation using acetonitrile, the post treatment samples were analysed on a C₁₈ column interfaced with a Triple Quadropole Tandem Mass Spectrometer. Positive electrosprayionization was employed as the ionization source. The mobile phase consisted of 0.1% formic acid–acetonitrile (60:40, v/v). Ciprofloxacin (CIPRO) was used as an internal standard. The analyte and internal standard (CIPRO) were monitored in the multiple reaction monitoring mode (MRM). The mass transition ion-pair has been followed as m/z 402→358.2 for MOXI and 332→288.1 for CIPRO. The method was linear in the concentration range of 25–5000 ng/mL. The lower limit of quantification was 25 ng/mL. The intra- and inter-day precision (relative standard deviation) and accuracy (relative error) values were within 12.4%. Each plasma sample was analyzed within 3 min.