| 5-Fu对肝癌细胞内凋亡抑制蛋白Survivin的诱导作用 |
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| 引用本文： | 罗顺峰,朱虹,张万广,张必翔,陈孝平.5-Fu对肝癌细胞内凋亡抑制蛋白Survivin的诱导作用[J].肝胆外科杂志,2002,10(3):227-228. |
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| 作者姓名： | 罗顺峰 朱虹 张万广 张必翔 陈孝平 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院肝脏外科中心,武汉,430030 |
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| 摘 要： | 目的 研究化疗药物5-Fu对肝癌细胞内凋亡抑制蛋白Survivin的诱导作用，探讨Survivin在肝细胞癌化疗抵抗中的作用机制。方法 培养人肝癌细胞株HepG2,加入低浓度的化疗药物5-Fu ，分别在加药前，加药后24h和48h采用逆转录聚合酶链反应（RT-PCR）和免疫印迹技术（western-blot)检测Survivin mRNA和蛋白的表达。结果 给予低浓度的5-Fu可诱导SurvivinmRNA和蛋白的表达发生变化，其中mRNA的加药后24h和48h分别较加药前提高了1.2倍和3.9倍，蛋白在加药后24h和48h分别较加药前提高了0.9倍和4.7倍。结论 5-Fu可诱导肝癌细胞内Survivin的表达增加，抵抗化疗药物诱导的细胞凋亡。
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| 关 键 词： | 肝细胞癌 survivin 5－Fu 凋亡抑制蛋白 |
| 文章编号： | 1006-4761(2002)03-0227-02 |
| 修稿时间： | 2001年10月20 |
THE EXPRESSION OF INHIBITOR OF APOPTOSIS PROTEIN OF SURVIVIN INDUCED BY 5-Fu IN HEPATOCELLUAR CARCINOMA(HCC) |
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| Luo Shunfeng,Zhu Hong,Zhang Wanguang,Zhang Bixiang,Chen Xiaoping.THE EXPRESSION OF INHIBITOR OF APOPTOSIS PROTEIN OF SURVIVIN INDUCED BY 5-Fu IN HEPATOCELLUAR CARCINOMA(HCC)[J].Journal of Hepatobiliary Surgery,2002,10(3):227-228. |
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| Authors: | Luo Shunfeng Zhu Hong Zhang Wanguang Zhang Bixiang Chen Xiaoping |
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| Institution: | Luo Shunfeng,Zhu Hong,Zhang Wanguang,Zhang Bixiang,Chen Xiaoping.Center of Hepatic Surgery,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China |
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| Abstract: | Objective To investigate the expression of survivin induced by chemotherapy agent(5 Fu),and determine the role in anti chemotherapy of hepatocellular carcinoma(HCC).Methods Low concentration of 5 Fu was added in cultured hepatocarcinoma cell line(HepG 2).The expression of survivin was detected by RP PCR and western blot on 24h and 48h after adding 5 Fu.Results Survivin mRNA level was increase 1.2 and 3.9 multiple,and the protein expression was increased 0.9 and 4.7 multiple.Conclusion A high expression of survivin was induced in HepG 2 by 5 Fu,and resist apoptosis induced by chemotherapy agent. |
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| Keywords: | Hepatocellular carcinoma Survivin 5 Fu |
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