Use of a ribosomal RNA gene probe for the epidemiological study of methicillin and ciprofloxacin resistantStaphylococcus aureus |
| |
| Authors: | K. Hadorn W. Lenz F. H. Kayser I. Shalit C. Krasemann |
| |
| Affiliation: | (1) Institute of Medical Microbiology, Gloriastr. 32, University of Zürich, CH-8028 Zürich, Switzerland;(2) Institute of Medical Microbiology and Immunology, University of Bonn, D-5300 Bonn, FRG;(3) Tel-Aviv Sourasky Medical Center, Infectious Disease Unit, IL-64239 Tel-Aviv, Israel;(4) Bayer AG, Pharma-Forschungszentrum, Institut für Chemotherapie, D-5600 Wuppertal 1, FRG |
| |
| Abstract: | Conventional bacteriophage typing was combined with ribotyping in the analysis of methicillin and ciprofloxacin resistantStaphylococcus aureus strains isolated in increasing frequency since the introduction of the new 4-quinolones as therapeutic agents in the Tel-Aviv Medical Center. Whole-cell DNA was digested withEcoRI andHindIII restriction endonucleases. Agarose gel electrophoresis, Southern blotting, and hybridization by biotinylated probe DNA coding for ribosomal RNA revealed 7 to 14 bands. Analysis of the patterns established a single DNA type inEcoRI as well as inHindIII digests for all strains except one. Control strains from other sources differed in their band patterns. Bacteriophage typing confirmed the results of DNA typing. Thus, the frequent occurrence of staphylococcal isolates resistant to 4-quinolones in the hospital was not due to mutational development of resistance in many strains, but to the spread of a resistant strain. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
