四氢呋喃与牛血清白蛋白相互作用的荧光光谱法研究

目的研究四氢呋喃（tetrahydrofuran,THF）与牛血清白蛋白（bovine serum albumin,BSA）相互作用的机制,探讨温度对其反应的影响。方法移取的BSA于石英比色皿中,搅拌混合均匀,避光静置,分别在25℃（298 K）、29℃（302 K）、33℃（306 K）、37℃（310 K）下以280 nm为激发波长,在LS-55荧光分光光度计上扫描280~500 nm波长范围内的发射光谱。结果不同温度下的结合猝灭常数（Ksv）为（3.466~6.317）×104L/mol,猝灭常数随温度的升高而减小。结论四氢呋喃对BSA的猝灭作用是属于静态猝灭。

Study on interaction between tetrahydrofuran and bovine serum albumin by fluorescence spectroscopy

[Objective]To study the mechanism of the interaction between tetrahydrofuran（ THF） and bovine serum albumin（ BSA）,probe the influence on the action caused by temperature.[Methods]BSA was moved into quartz colorimetric dish,mixed with a certain amount of THF,and let stand in dark condition. The emission spectrum in 280 ~500 nm wavelength range was scanned using the LS-55 fluorescence spectrophotometer at 25 ℃（ 298 K）,29 ℃（ 302 K）,33 ℃（ 306 K） and 37 ℃（ 310 K） with the excitation wavelength of 280 nm.[Results]The quenching constants（ Ksv） at different temperature were between 3. 466 ×104L/mol and 6. 317 ×104L/mol,and decreased with increasing temperature.[Conclusion]The fluorescence quenching of BSA by THF is identified as the static quenching.