| ICAM-1在缺氧-再氧化引起的白细胞内皮细胞粘附中的作用 |
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| 作者姓名： | Qian G Liu H Zhao Y |
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| 作者单位： | 中国医学科学院中国协和医科大学血液学研究所止血血栓与血管研究室,天津,300020 |
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| 基金项目： | 国家自然科学基金(39370301)资助 |
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| 摘 要： | 目的探讨细胞间粘附分子(ICAM-1是否介导缺氧-再氧化引起的中性粒细胞(PMN)和血管内皮细胞(VEC)的粘附反应.方法血管内皮细胞(VEC)经缺氧再氧化(H/R)处理后,加入PMN,用计数法检测粘附率,以细胞免疫化学及原位杂交法检测ICAM-1及ICAM-1mRNA表达.结果VEC经H/R处理后,PMN与其粘附率增高1倍(P＜0.01),ICAM-1单克隆抗体(mAb)与CD11a/CD18mAb可明显降低粘附率的增高,H/R能增加VEC的ICAM-1及ICAM-1mRNA表达.结论ICAM-1介导H/R后的PMN-VEC间粘附反应.
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| 关 键 词： | 血管内皮细胞 缺氧再氧化 细胞间粘附分子 |
| 修稿时间： | 1997年7月15日 |
The role of ICAM-1 in leukocyte adherence to endothelial cells induced by hypoxia/reoxygenation |
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| Qian G,Liu H,Zhao Y.The role of ICAM-1 in leukocyte adherence to endothelial cells induced by hypoxia/reoxygenation[J].Acta Academiae Medicinae Sinicae,1999,21(2):130-134. |
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| Authors: | Qian G Liu H Zhao Y |
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| Institution: | Department of Hemostasis, Thrombosis and Vasculature, Institute of Hematology, CAMS and PUMC, Tianjin 300020. |
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| Abstract: | OBJECTIVE: To determine whether intercellular adhesion molecule-1 (ICAM-1) mediats the polymorphonuclear leukocyte (PMN) adherence to endothelial cells elicited by hypoxia/reoxygenation (H/R). METHODS: Human umbilical vein endothelial cells (HUVEC) monolayers was exposed to hypoxia for 30 min or 120 min then reoxygenated. Count the number of PMN in adhesion assays. Expression of ICAM-1 and ICAM-1 mRNA was detected by immunohistochemical analysis and in situ hybridization. RESULTS: PMN adhesion to HUVEC exposed to H/R was significantly increased (1 fold) as compared with that of control (P < 0.01). H/R induced hyperadherence was significantly diminished by monoclonal antibodies directed at ICAM-1 or CD11a/CD18. The results showed an increased expression of ICAM-1 and ICAM-1 mRNA on HUVEC exposed to H/R. Hypoxic HUVEC might be responsible for PMN adesion. CONCLUSIONS: ICAM-1 mediated the adhesive interaction between PMN and VEC induced by hypoxia/reoxygenation. |
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