| 辣椒素诱导白血病和肝癌细胞凋亡的比较研究 |
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| 引用本文： | 陈媛媛,陈静,易娟,魏虎来.辣椒素诱导白血病和肝癌细胞凋亡的比较研究[J].中药药理与临床,2008,24(6). |
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| 作者姓名： | 陈媛媛 陈静 易娟 魏虎来 |
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| 作者单位： | 兰州大学基础医学院医学实验中心,甘肃省新药临床前研究重点实验室,兰州,730000 |
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| 基金项目： | 甘肃省新药临床前研究重点实验室资助项目，甘肃省天然药物重点实验室开放基金 |
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| 摘 要： | 目的:比较合成辣椒素(N-Vanillylnonanamide)诱导白血病细胞K562及其多药耐药K562/ADM、肝癌HepG2细胞凋亡的作用.方法:以K562细胞、K562/ADM细胞和HepG2细胞为靶细胞,采用噻唑蓝(MTT)比色法检测细胞增殖活性,流式细胞法测定细胞周期和Annexin V/PI双染色检测细胞凋亡.结果:MTT示合成辣椒素可抑制白血病细胞和HepG2细胞的增殖,根据同时期半数抑制浓度(IC50)示HepG2细胞最敏感,K562细胞较K562/ADM细胞敏感.合成辣椒素可阻滞细胞周期.合成辣椒素诱导24小时后Annexin V/PI双染色显示白血病细胞和肝癌细胞凋亡率升高;白血病敏感细胞株和耐药细胞株的对舍成辣椒素敏感性相似.结论:合成辣椒素可抑制白血病和肝癌细胞的增殖并诱导其凋亡;辣椒素对药物敏感和耐受的白血病细胞的作用相似.
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| 关 键 词： | 辣椒素 凋亡 白血病 肝癌 |
Comparative study on apoptosis of leukemia and hepatic tumor cells induced by synthetical capsaicin N-Vanillylnonanamide |
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| Chen Yuanyuan,Chen Jing,Yi Juan,Wei Hulai.Comparative study on apoptosis of leukemia and hepatic tumor cells induced by synthetical capsaicin N-Vanillylnonanamide[J].Pharmacology and Clinics of Chinese Materia Medica,2008,24(6). |
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| Authors: | Chen Yuanyuan Chen Jing Yi Juan Wei Hulai |
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| Institution: | Chen Yuanyuan,Chen Jing,Yi Juan,Wei Hulai(Laboratory Center for Medical Science,School of Basic Medical Sciences,Lanzhou University,Key Laboratory of Preclinical Study for New Drugs of Gansu Province,Lanzhou 73000) |
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| Abstract: | Objective :To compare the apoptosis-inducing effects of synthetical capsaicin(N-Vanillylnonanamide)on leukemia cells and hapatic tumor cells.Methods:The K562 cells,K562/ADM cells and HepG2 cells were used as the target cells,The cell proliferating activity was assessed with a MTT colorimetric assay,the cell cycle was measured using flow cytometry,and the cellular apoptosis was investigated by Annexin V/PI staining.Results:N-Vanillylnonanamide was able to inhibit the proliferation of MTT in leukemia cells an... |
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| Keywords: | N-Vanillylnonanamide apoptosis leukemia hapatic tumor |
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