吲哚-3-原醇对乙醇损伤性大鼠肝切片的保护作用

目的　采用精密肝切片技术 ,研究十字花科类蔬菜提取物吲哚 3 原醇 (I3C)对乙醇肝损伤的作用及机制。方法　制作大鼠乙醇损伤肝切片模型 ,观察不同剂量I3C对培养液中肝损伤标志酶及肝细胞浆苯胺羟化酶 (ANH)、乙醇脱氢酶 (ADH)活性的影响 ,并进行组织学检查。结果　乙醇5 0mmol·L-1作用肝切片 4h时 ,培养液谷丙转氨酶、谷草转氨酶、乳酸脱氢酶和谷胱甘肽S 转移酶活性明显升高 ,同时肝细胞浆ANH活性升高、ADH活性降低 ;加入 10 0～ 4 0 0μmol·L-1的I3C后 ,培养液中各酶活性降低的同时 ,肝细胞ANH和ADH活性恢复正常。肝切片病理学检查也证实I3C的保护作用。结论　I3C能有效拮抗乙醇所致的肝损伤 ,其机制与改变乙醇代谢途径有关。

Protection of indole-3-carbinol against ethanol-induced hepatotoxicity in precision liver-cut slices of rats

AIM To study the hepato-protection of indole-3-carbinol (I3C), one of glucosinolate compounds in cruciferous vegetables, on ethanol-induced hepatotoxicity. METHODS Ethanol 50 mmol·L -1 was used to induce hepatotoxicity in precision liver-cut slices of rats. The release of glutamic pyruvic transaminase(GTP), glutamic oxaloacetic transaminase (GOT), lactate dehydrogenase (LDH) and glutathione S-transferase (GST) into culture medium, and aniline hydroxylase (ANH) and alcohol dehydrogenase (ADH) in cytosol of hepatocytes in the precision liver-cut were measured with different doses of I3C. The histopathological observation of liver slices was also done to evaluate the effect of I3C. RESULTS The release of GTP, GOT, GST and LDH in the medium increased significantly when the slices were treated with 50 mmol·L -1 ethanol for 4 h. I3C at concentrations of 100～400 μmol·L -1 effectively reduced the release of GTP, GOT, GST and LDH in the medium of ethanol-treated slices. Meanwhile, the activities of cytosol ANH and ADH decreased to the normal levels. The histopathological changes of liver slices induced by ethanol were lessened by I3C treatment. CONCLUSION I3C can markedly protect rat liver slices from ethanol-induced hepatotoxicity, which partly results from the change of ethanol metabolism in liver.