Phenotypic characterization ofTheileria parva schizonts by two-dimensional gel electrophoresis |
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| Authors: | C. Sugimoto P. A. Conrad L. Mutharia T. T. Dolan W. C. Brown B. M. Goddeeris T. W. Pearson |
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| Affiliation: | (1) International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya;(2) Department of Biochemistry, University of Nairobi, P.O. Box 30197, Nairobi, Kenya;(3) Department of Biochemistry and Microbiology, University of Victoria, V8W 2Y2 Victoria, British Columbia, Canada;(4) Present address: Department of Veterinary Microbiology and Immunology, University of California, 95616 Davis, CA, USA;(5) Present address: Department of Veterinary Microbiology and Parasitology, Texas A & M University, 22843-4426 College Station, TX, USA |
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| Abstract: | Biosynthetically radiolabelledTheileria parva schizonts were purified from bovine lymphoblastoid cells and their proteins were analyzed by two-dimensional gel electrophoresis and autoradiography. The protein spot patterns of schizont proteins from three stocks ofT. parva parva indicated that the phenotypic diversity among the stocks was minimal, with the Mariakani and Uganda stocks being identical and the Muguga stock showing only a few differences in minor spots. Comparison of the spot patterns of schizonts of threeT. parva subspecies showed thatT. p. parva andT. p. bovis differed in only one protein and thus could not be reliably distinguished on the basis of their protein differences. However,T. p. lawrencei showed several protein differences and could be distinguished easily from the other subspecies. Differences in schizont-protein spot patterns were also seen when two different cell lines were infected with the sameTheileria stabilate, when one cell line was infected with two different stabilates of the same stock and when uncloned and cloned infected cell lines were used. These results suggest the possibility that selection of phenotypically different parasites could occur in vivo or in vitro.II RAD publication 641 |
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