25例肝癌中p16基因状态的研究

目的：研究原发性肝细胞性肝癌中p１６基因的状态。方法：采用多重ＰＣＲ分析法、ＳＳ ＣＰ分析法及以ＰＣＲ为基础的甲基化分析法分别对四川地区人群２５例原发性肝细胞性肝癌标本及其相庆的癌旁组织中p１６基因的缺失、突变及甲基化情况进行检测。结果：２５例肿瘤标本中，发现３例p１６基因缺失，其中２例纯合子缺失，１例半合子缺失，未发现 １例突变，在未发现基因缺失的２２例肿瘤中发现６例有p１６基因高甲基化存在。全部相

The status of p16 gene in primary hepatocellular carcinoma from a Chinese population

Objective: To investigate the status of p16 gene in primary hepatocellular carcinoma (HCC). Methods: A total of 25 HCC samples with correspondant tumor-adjacent specimens were screened for p16 gene deletions, mutations and hypermethylations, by using multiplex polymerase chain reaction (PCR), single strand conformational polymorphism(SSCP) and PCR based methylation analysis, respectively. Results: Among 25 HCC samples, 2 homozygous and 1 hemizygous deletions and 6 hypermethylations were detected and no mutations were idendtified. However, no deletions, mutations or hypermethylations were found in all the tumor-adjacent tissues. Conclusion: The abnormal alterations of p16 gene may play an important role in the carcen genesis of primary hepatocellular carcinoma.