高效液相色谱法测定闭鞘姜属3种植物中总薯蓣皂苷元的含量

目的测定姜科闭鞘姜属植物闭鞘姜Costus speciosus、莴笋花C.lacerus、光叶闭鞘姜C.tonkinensis根茎中总薯蓣皂苷元(Diosgenin)的含量。方法用HPLC法，分析柱为C8柱(5μm，4.6mm×250mm)，乙腈-水(80∶20)为流动相，检测波长205nm。结果闭鞘姜、莴笋花、光叶闭鞘姜含薯蓣皂苷元分别为1.4734%、0.3128%、0.4911%。薯蓣皂苷元平均回收率102.3%，RSD为3.8%(n=5)。结论三种闭鞘姜属植物中薯蓣皂苷元的含量差异较大，应合理开发利用。

Determination of Diosgenin in Three Species of Costus By HPLC

Objective:HPLC was used to determine the contents of diosgenin in rhizomes of Costus speciosus, C.lacerus and C.tonkinensis.Methods:The column was Alltima C8 column(5 μm, 4.6 mm×250 mm). The mobile phase consisted of acetonitrile-water (80∶20). The determination wavelength was 205 nm. Results: The amount of diosgenin is 1.473 4% for Costus speciosus; 0.312 8% for C. lacerus;0.491 1% for C. tonkinensis respectively. The average recovery of diosgenin is 102.3%, RSD 3.8% (n=5).Conclusion:Three plants of the Costus genus contain different amount of diosgenin. They should be developed and applied reasonably.