| PTEN在支气管哮喘大鼠气道炎症中的作用 |
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| 引用本文： | 徐慧,戴元荣,曾潍贤. PTEN在支气管哮喘大鼠气道炎症中的作用[J]. 中华哮喘杂志(电子版), 2010, 4(1): 20-23 |
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| 作者姓名： | 徐慧 戴元荣 曾潍贤 |
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| 作者单位： | 温州医学院附属二院呼吸内科,325003 |
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| 摘 要： | 目的探讨PTEN在支气管哮喘（简称哮喘）人鼠气道炎症中的作用。方法20只SPF级雄性SD大鼠随机分为2组。对照组和哮喘组。以卵清白蛋白致敏激发法复制大鼠哮喘模型，每只大鼠左肺留取肺组织，右肺进行支气管肺泡灌洗歼留取支气管肺泡灌洗液（BALF）。对BALF进行细胞分类计数；应用双抗体夹心酶联免疫吸附试验（Sandwich ELISA）法测定BALF中IL-4、IL-12浓度；采用免疫组织化学法和Western blot法测定PTEN蛋白的表达和量的变化。结果①BALF IL-4的浓度哮喘组显著高于对照组，BALF中IL-12的浓度哮喘组屁著低于对照组（P值均〈0．01）；②免疫组织化学显示PTEN蛋白主要表达细胞是支气管上皮细胞，Western blot法显示哮喘组支气管PTEN蛋白的表达显著低于对照组（P〈0．01）；③支气管上皮细胞PTEN蛋白表达量分别与BALF中的IL-4浓度呈显著负相关，与BALF中的IL—12浓度呈显著正相关。结论哮喘大鼠PTEN蛋白表达明显减少，它能减少Th2因子的表达，可能与哮喘大鼠气道炎症中Th1／Th2平衡密切相关。
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| 关 键 词： | PTEN 哮喘 气道炎症 |
Effect of PTEN on airway inflammation in a rat bronchial asthma model |
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| XU Hui,DAI Yuan-rong,ZENG Wei-xian.Departmeat of Respiratory Medicine,the Second Affiliated Hospital of Wenzhou Medical College,Wenzhou China. Effect of PTEN on airway inflammation in a rat bronchial asthma model[J]. Chinese Journal of Asthma(Electronic Version), 2010, 4(1): 20-23 |
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| Authors: | XU Hui DAI Yuan-rong ZENG Wei-xian.Departmeat of Respiratory Medicine the Second Affiliated Hospital of Wenzhou Medical College Wenzhou China |
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| Affiliation: | XU Hui,DAI Yuan-rong,ZENG Wei-xian.Departmeat of Respiratory Medicine,the Second Affiliated Hospital of Wenzhou Medical College,Wenzhou 325003 China |
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| Abstract: | Objective To study the effect of PTEN on airway inflammation in a rat bronchial asthma (asthma) model.Methods Twenty Male Sprague-Dawley rats were radomly divided into control group and asthma group.The lung tissue was sampled from the left lung.Bronchoalveolar lavage fluid(BALF)was collected from the right lung.The concentration of the IL-4 and IL-12 in BALF were measured by Sandwich ELISA.The PTEN protein was observed by the technique of immunohistochemistry and the level of PTEN in lung homogenate was me... |
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| Keywords: | PTEN Asthma Airway inflammation |
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