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| 受体相关蛋白对实验性自身免疫性重症肌无力的乙酰胆碱受体的作用 | |
| 引用本文： | 张旭,Mario Losen,Marc De Baets. 受体相关蛋白对实验性自身免疫性重症肌无力的乙酰胆碱受体的作用[J]. 中国免疫学杂志, 2003, 19(9): 628-630 |
| 作者姓名： | 张旭 Mario Losen Marc De Baets |
| 作者单位： | 1. 温州医学院附属第一医院神经科,温州,325000 2. 荷兰马斯特利赫特大学免疫学系 3. 荷兰马斯特利赫特大学神经病学系 |
| 基金项目： | 本工作在欧洲重症肌无力中心,欧洲神经生物学博士后流动站,荷兰马斯特利赫特大学神经病学系完成 |
| 摘 要： | 目的：探讨受体相关蛋白(Rapsyn)对实验性自身免疫性重症肌无力(EAMG)动物终板乙酰胆碱受体的作用。方法：用基因工程的方法制备提取pcDNA-Rapsyn，并将其注入动物肌肉左大腿外侧，右大腿相应部位注射相同剂量的空白质粒(Vector)，以方波刺激使其进入细胞膜内，2周后用单克隆抗体35(mAb35)诱导出EAMG模型并进行症状评估，mAb35诱导48小时后取双大腿外侧肌，用放射免疫法检测AChR，并计算出AChR的损失率。结果：在EAMG模型中，被pcDNA-Rapsyn预处理的肌肉AChR损失率明显低于未经处理的肌肉。结论：Rapsyn蛋白对EAMG模型的AChR有保护作用。 |
| 关 键 词： | 受体相关蛋白 实验性自身免疫性重症肌无力 乙酰胆碱受体 作用 |
| 文章编号： | 1000-484X(2003)09-0628-03 |
The effect of receptor-associated protein in acetylcholine receptor of experimental autoimmune myasthenia gravis |
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| Mario Losen,Marc De Baets. The effect of receptor-associated protein in acetylcholine receptor of experimental autoimmune myasthenia gravis[J]. Chinese Journal of Immunology, 2003, 19(9): 628-630 | |
| Authors: | Mario Losen Marc De Baets |
| Abstract: | Objective:To investigation the effect of receptor associated protein (Rapsyn) in acetylcholine receptor (AChR) of end plate of experimental autoimmune myasthenia gravis (EAMG).Methods:Made and purified the pcDNA Rapsyn with genetic engineering technique and injected the muscle of vastus lateralis of left leg of Lewis rats, and then stimulated with electro square porator. EAMG induced by passive transfer mAb35 injected intraperitoneally, and monitor body weight and assessment clinical signs score. Sacrificed animal and removed the muscle of vastus lateralis of Lewis rats after 48 hour of induction of EAMG, measurement the AChR with radioimmunoassay (RIA) and calculated the AChR loss rate.Results:There were lower AChR loss rate found in muscle pretreated with pcDNA Rapsyn then muscle pretreated with Vector(as negative control).Conclusion:Rapsyn protein can protect the AChR of EAMG model. [ |
| Keywords: | Receptor associated protein Experimental autoimmune myasthenia gravis Acetylcholine receptor |
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