An adenosine triphosphate bioluminescence assay for detecting the number of living cells] |
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| Authors: | S Liu Z Peng H Wang J Lou B He Q Tang D Qiu |
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| Affiliation: | Department of Obstetrics and Gynecology, Second Affiliated Hospital, WCUMS, Chengdu 610041. |
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| Abstract: | The method for detecting the number of living cells was studied. Using an adenosine triphosphate (ATP) bioluminescence assay, the present authors reported a perfect linear relationship between lg ATP concentrations and lg luminescence counts (r = 0.9963) as well as a relationship between lg number of cells and lg ATP luminescence counts (r = 0.9922). The detectable cells ranged from 10(2) to 10(6) cells/ml, the coefficients of variation 1-3%. This method is simple, accurate and sensitive and has a high reproducibility. |
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