| 茸毛链球菌主要表面蛋白抗原的定性分析 |
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| 引用本文： | 张平,樊明文,边专,杜民权,刘宁慧. 茸毛链球菌主要表面蛋白抗原的定性分析[J]. 口腔医学研究, 1999, 15(1): 8-10 |
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| 作者姓名： | 张平 樊明文 边专 杜民权 刘宁慧 |
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| 作者单位： | 武汉市,430070,湖北医科大学附属口腔医学院 |
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| 摘 要： | 目的：提取茸毛链球菌主要表面蛋白抗原（ＰＡｇ）对其性质进行初步鉴定。方法：用硫酸铵沉淀、ＤＥＡＥ－ｃｅｌ－ｌｕｌｏｓｅ离子交换和 Ｓｅｐｈａｒｏｓｅ ６Ｂ凝胶过滤从茸毛链球菌 ６７１５培养上清中提取 ＰＡｇ，并对其分子量，蛋白质含量，氨基酸组成及免疫学特性进行检测。结果与结论：ＰＡｇ能与特异性抗血清发生反应，ＳＤＳ－ＰＡＧＥ呈单一区带，分子量为２１０ＫＤ，蛋白质含量为６１．４％，氨基酸组成分析揭示其酸性和碱性氨基酸分别为２６．７％和１０．７％。
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| 关 键 词： | 主要表面蛋白 茸毛链球菌 纯化 |
| 修稿时间： | 1998-10-20 |
Purification and characterization of the major cell surface protein antigen of streptococcus sobrinus |
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| Zhang Ping, Fan Mingwen, Bian Zhuan. et al. Purification and characterization of the major cell surface protein antigen of streptococcus sobrinus[J]. Journal of Oral Science Research, 1999, 15(1): 8-10 |
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| Authors: | Zhang Ping Fan Mingwen Bian Zhuan. et al |
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| Abstract: | Objective: To purify and characterize the major cell surface protein antigen of streptococcus sobrinus. Methods: PAg was purified by 65 % saturate ammonium sulfate precipitation, DEAE - cellulose ion - exchange chromatography and Sepharose 6B gel - filtration chromatography. Results and Conclusion: SDS-PAGE showed the extract PAg was pure and in the ranges of 210KD, protein quantitative analysis showed PAg contained 61.4 % proteins, amino acid analysis revealed that PAg contained 26. 7 % acidic and 10.7 % basic acid residues. |
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| Keywords: | PAg streptococcus sobrinus purification |
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