| HPLC法测定夜香牛中木犀草素的含量 |
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| 引用本文： | 陈黄保. HPLC法测定夜香牛中木犀草素的含量[J]. 中国药师, 2009, 12(9): 1262-1263 |
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| 作者姓名： | 陈黄保 |
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| 作者单位： | 湛江市药品检验所,广东湛江,524037 |
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| 摘 要： | 目的：建立夜香牛中木犀草素的含量测定HPLC方法。方法：色谱柱为Agilent C18（150mm×4．6mm，5μm），甲醇-0．2％磷酸溶液（44：56）为流动相，流速1．0ml·min^-1，检测波长350m。结果：木犀草素在0．93～9．27μg·ml^-1（r=0．9998）范围内呈良好线性关系，平均回收率为99．2％，RSD=1．7％（n=6）。结论：方法简便，准确，重复性好。可有效地控制夜香牛的质量。
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| 关 键 词： | 夜香牛 木犀草素 高效液相色谱法 |
Determination the Content of Luteolin in Vernonia Cinerea by HPLC |
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| Chen Huangbao. Determination the Content of Luteolin in Vernonia Cinerea by HPLC[J]. China Pharmacist, 2009, 12(9): 1262-1263 |
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| Authors: | Chen Huangbao |
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| Affiliation: | Chen Huangbao ( Zhanjiang Institute for Drug control, Guangdong, Zhanjiang 524037, China) |
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| Abstract: | Objective： To establish a HPLC method for determination the content of luteolin in vemonia cinerea. Method： The Agilent C18 column （ 150mm×4.6mm,5μm） was used, the methanol - 0. 2% phosphoric acid solution （ 44 ： 56 ） was mobile phase, the flow rate was 1.0ml·min^- 1, the detection wavelength was 350nm. Result： The linear range of luteolin was 0.93 -9.27 μg·ml^-1, r = 0.999 8 ,the average recovery was 99.2% with RSD 1.7% （ n = 6）. Conclusion： The method is simple, accurate and repeatable, it can be used for control the quatity of vernonia cinerea. |
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| Keywords: | Vernonia Cinerea Luteolin HPLC |
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