非小细胞肺癌Fas/FasL蛋白的表达及临床意义

目的：研究Fas／FasL蛋白在非小细胞肺癌（NSCLC）的表达及其临床意义。方法：应用链霉菌抗生物素蛋白2过氧化物酶（SP）法染色技术测定65例NSCLC组织标本Fas／FasL蛋白的表达水平，并分析其与病理类型、区域淋巴结转移、病理分级及TNM分期之间的关系。结果：NSCLC组织中Fas蛋白表达率显著低于正常肺组织（46．15％vs80．00％，P〈0．05），而FasL蛋白表达率显著高于正常肺组织（49．23％vs13．33％，P〈0．05）；FasL的表达与肺癌区域淋巴结转移有关，但Fas/FasL与肿瘤病理类型、病理分级及TNM分期无关。结论：Fas／FasL蛋白可能参与了非小细胞肺癌细胞凋亡的调节；检测FasL蛋白的表达有可能为临床判断NSCLC区域淋巴结转移、病变的发展以及评估预后提供依据。

Expression of Fas/Fasl Gene Protein and its Chinical Significance in Human Non-small Cell Lung Cancer

Objective: To study the clinical significance of the expression of Fas/FasL gene protein in non-small cell lung cancer(NSCLC).Methods: By using SP immuno-histochemical method with anti-Fas and FasL multi2clonal antibody,we detected the expression of Fas/FasL gene protein in 65 cases of NSCLC and studied it s correlation with histological types,local lymph node metastases,pathological grades and TNM stages.Results: The positive expression rate of Fas(46.15%) in NSCLC was much lower than that(80.00%) in the normal lung tissues(P< 0.05),but the expression rate of FasL(49.23%) in NSCLC was much higher than that(13.33%) in the normal lung tissues(P< 0.05).FasL was correlated with the stage of local lymph node metastases,but none of them had any correlation with histological types,patholo-gical grades or the TNM stages(P>0.05).Conclusions: The data demonst rated that Fas/FasL might involve in the regulation of apoptosis of NSCLC,and the expression of FasL might be used as an objective indicator of the metastatic potency of local lymph-node,extent of invation and the prognosis in NSCLC.