CyclinD1 c-myc和p53的表达与肝细胞癌生物学行为关系

目的:探讨肝细胞癌(Hepatocellularcarcinoma,HCC)及癌旁肝组织CyclinD1、c-myc及p53蛋白表达的意义及其与肝癌生物学行为关系。方法:采用EnVisionTMplus免疫组织化学方法检测CyclinD1、c-myc及p53蛋白在52例HCC及癌旁肝组织中的表达。结果:CyclinD1及c-myc在HCC组织中的阳性率明显高于在癌旁肝组织中的阳性率(P<0.05),在HCC中p53的阳性表达与CyclinD1、c-myc的阳性表达呈正相关(r=0.4637,P=0.0022、r=0.3445,P=0.0273,);CyclinD1、c-myc及p53在人肝癌组织中的检出率与肝外转移、术后复发及肿瘤分化程度明显有关(P<0.05),CyclinD1的检出率与门静脉癌栓明显有关(P<0.05),p53的检出率与血清AFP和TSGF水平有显著性差异(P<0.05);CyclinD1、c-myc及p53的检出率与临床分期、肿瘤大小、肿瘤个数、HBsAg状况无明显关系。结论:CyclinD1、c-myc及p53蛋白的过表达可促使肝癌细胞增殖,使肝癌细胞具有更强的侵袭力,与肝癌的发生、发展密切相关。

The Relationship between the Expression of CyclinD1, c-myc and p53 Genes and the Biological Behavior of Hepatocellular Carcinoma

Objective: To evaluate the correlation between the expression of CyclinD1, c-myc and p53 genes and the biological behavior of hepatocellular carcinoma (HCC). Methods: Immunohistochemical technology (EnVisionTM plus) was applied to detect the expression of CyclinD1, c-myc and p53 in 52 HCC tissues and their adjacent liver tissues. Results: The positive rates of CyclinD1 and c-myc were significantly higher in the HCC tissue than those of adjacent liver tissue (P<0.05). There was a positive correlation between the expression of p53 and CyclinD1 and c-myc in the HCC tissue (r =0.4637, P=0.0022, r=0.3445, P=0.0273, respectively). The positive rates of CyclinD1, c-myc and p53 in the HCC tissue were significantly correlated with the presence of extrahepatic metastasis, the recurrence of tumor and the differentiation of tumor (P<0.05). The positive rate of CyclinD1 was significantly correlated with the ortal vein tumor thrombus (P<0.05), The positive rate of p53 was significantly correlated with the level of serum alpha-fetoprotein (AFP) and tumor specific growth factor (TSGF) (P<0.05). The positive rates of CyclinD1, c-myc and p53 were not correlated with the clinical stage, the diameter of tumor, the number of tumor and the level of serum HBsAg. Conclusions: The over-expression of CyclinD1, c-myc and p53 proteins may contribute to the proliferation of hepatoma cell, improve thegressive ability of hepatoma cell, and relates to the initiation and progression of HCC.