一种新型大鼠胰腺移植模型的建立

目的：建立大鼠同种异体胰腺移植模型。为进一步进行胰腺移植的基础研究创造条件。方法：采用大鼠颈部作为移植部位，不阻断体循环，运用显微外科技术，将供胰附带的主动脉段与受体颈总动脉以袖套法吻合，供胰的门静脉与受体的颈外静脉行端端吻合，术后注意补液与保温。结果：共进行70次大鼠胰腺移植手术，其中正式实验20次，供受体手术时间均约1h，手术成功率为85%；移植胰的组织学变化与临床胰腺移植相似。结论：本胰腺移植模型操作简便、结果稳定，并能较好地模拟临床胰腺移植过程，适用于该领域的基础研究。

The Establishment of a Novel Rat Model of Pancreas Transplantation

Objective: To establish a rat model of pancreas transplantation for basic research.Methods: The neck region of rat was selected as transplanting site and the operation was performed without clamping the systemic circulation.The aortic segment of the graft was anastomosed with recipient's cervical artery by cuff technique,while the portal vein of the graft was anastomosed with recipient's jugular vein by microsurgical sewing.Fluid supplement and warm keeping was maintained after transplantation.Results: Seventy experiments were performed,among which 20 were former transplantation.The total procedure of transplantation lasted about 1 hour and success rate was 85%.The histological change was similar to those of clinical pancreas transplantation.Conclusion: This pancreas transplantation model is simple and stable,while imitating the clinical procedure perfectly.It can be utilized in basic research of pancreas transplantation.