腺苷A_(2a)受体激活对脑出血后炎症反应和细胞凋亡的影响

目的探讨脑出血(ICH)后激活腺苷A2A受体对中性粒细胞浸润、TNF-α蛋白的表达及细胞凋亡的影响。方法 SD大鼠随机分为对照组、ICH组、CGS组、CGS+ZM组、ZM组。应用胶原酶脑内立体定向注射制作ICH模型。对照组注射生理盐水2ul,CGS组、CGS+ZM组、ZM组在ICH模型制作前15min,脑内立体定向分别注射CGS1μg,CGS1μg+ZM1μg,ZM1μg。模型制作后24h处死动物,应用HE染色检测中性粒细胞的浸润,应用Western-Blot技术检测TNF-α蛋白的表达,应用TUNEL法检测细胞凋亡。结果 ICH组可见明显的中性粒细胞浸润、TNF-α蛋白的表达及凋亡的细胞,与对照组比较,差别具有显著性(P0.001)。CGS组中性粒细胞浸润、TNF-α的表达及凋亡的细胞数目明显降低,与ICH组比较,差别具有显著性(P0.001)。CGS+ZM组中性粒细胞浸润、TNF-α的表达及凋亡的细胞明显增高,与ICH组比较差异无显著性(P0.05),与CGS组比较差异具有显著性(P0.001)。结论选择性的腺苷A2a受体激活可降低ICH后的炎症反应和细胞凋亡,从而减轻ICH后组织的损伤。

Effect of adenosine receptor A2a activation on the inflammation and apoptosis after intracerebral hemorrhage

Objective To investigate the effect of adenosine receptor A2a activation on the neurophil cellular infiltration,expression of TNF-α protein and apoptosis after intracerebral hemorrhage（ICH）.Methods SD rats were randomly divided into control,ICH,CGS,CGS＋ZM,and ZM groups.ICH models were performed by collagenase stereotaxical injection.Control group were injected 2 ul physiologic saline.CGS,CGS＋ZM and ZM group were respectively injected CGS 1 μg,CGS 1 μg＋ZM 1 μg and ZM 1 μg at 15 minutes before ICH models were performed.Rats were put to death at 24 th hour after modeling.Neurophil cellular infiltration was detected by HE dyeing.Expression of TNF-α protein was determined by Western-blot method.Apoptosis was measured by TUNEL method.Results Neurophil cellular infiltration,expression of TNF-α protein and apoptosis in ICH group were increased and had significant difference compared with control group（P〈0.001）.Neurophil cellular infiltration,expression of TNF-α protein and apoptosis in CGS group were decreased and had significant difference compared with ICH group（P〈0.01）.Neurophil cellular infiltration,expression of TNF-α protein and apoptosis in CGS＋ZM group were incereased and had no significant difference compared with ICH group（P〉0.05）.Conclusions Selective adenosine receptor A2a activation can depress inflammation and apoptosis after cerebral hemorrhage,and might reduce tissue injury in or surround hemotoma.