| 钙超载大鼠心肌细胞核钙转运功能的改变 |
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| 引用本文： | 王培勇,李夏.钙超载大鼠心肌细胞核钙转运功能的改变[J].基础医学与临床,1999,19(2):30-33,39. |
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| 作者姓名： | 王培勇 李夏 |
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| 作者单位： | 北京医科大学心血管基础研究所病生室 |
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| 摘 要： | 目的：观察钙超载对大鼠心肌细胞核钙转运系统的影响。方法：大鼠心肌钙超模型采用腹腔注射ＶｉｔＤ３制备，心肌细胞核采用差束离心和密度梯度离心分离纯化，用酶的纯度及测定ＡＴＰａｓｅ活性，用＾４５Ｃａ＾２＋测定核钙摄取。结果：钙超载大鼠心肌钙含量与对照组相经增加４２％，核钙含量增加５５％，钙超载大鼠心肌细胞核Ｃａ－ＡＴＰａｓｅ活性降低，其Ｖｍａｘ降低２４％，Ｋｍ无显著改变；「＾４５Ｃａ＾２＋」摄取也无显著
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| 关 键 词： | 钙超载 心肌 细胞核 钙转运功能 心血管疾病 |
Effect of Calcium Overload on Myocardial NuclearCalcium Transport in Rats |
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| Wang peiyong, Li Xia, Yao Xinghai, Su Jingyi, Tang Jian, Tang Chaoshu,.Effect of Calcium Overload on Myocardial NuclearCalcium Transport in Rats[J].Basic Medical Sciences and Clinics,1999,19(2):30-33,39. |
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| Authors: | Wang peiyong Li Xia Yao Xinghai Su Jingyi Tang Jian Tang Chaoshu |
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| Abstract: | Objective:To investigate the effects of calcium overload on myocardial calcium transportsystem.Methods: myocardial rat calcium overload model was established by intraperitoned injectionof vitamin D3. Myocardial nuclei were purified by sucrose density gradient supercentrifugation andidentified enzymologically.The activity of Ca2 -ATPase was measured and calcium uptake wasassayed with asCa2 isotope.Results:The calcium content in myocardial homogenate and nuclei incalcium overload rats increased by 42% and 55% respectively(P<0.0l as compared with control) .Vmax of ATPase activity decreased by about 24%(P<0.05) , but Km remained unchanged.45Ca2 ]uptake also remained unchanged. Conclusion:The above results suggest that calciumoverload induced passive increase in myocardial nuclear calcium content. |
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| Keywords: | calcium overload myocardium Calcium nucleus |
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