石蜡包埋的石棉肺癌组织中p53基因突变的分析方法

为了利用石蜡包埋的病理组织来研究石棉肺癌的基因突变情况，选用１０例石蜡包埋的石棉肺癌组织进行ＰＣＲ－ＳＳＣＰ分析，检测其抗癌基因ｐ５３的第５、第７和第８外显子的突变情况。经银染检查，发现４个病例的ｐ５３基因的第７或第８外显子片段呈突变阳性。用放射性自显影的ＰＣＲ－ＳＳ－ＣＰ方法分析这１０例病例，检测到７个病例的ｐ５３基因第７或第８外显子片段呈突变阳性。用两种方法检测均未发现这１０个病例的第５外显子呈突变阳性。银染ＰＣＲ－ＳＳＣＰ检测可检出放射性自显影ＰＣＲ－ＳＳＣＰ分析结果的６０％，二者的结果相符合。因而，简便、灵敏而且危害性小的银染检测方法，可以代替放射性自显影用于ＰＣＲ－ＳＳＣＰ分析，研究石棉肺癌的基因突变情况。

Detection of p53 Gene Mutation in Paraffin embedded Asbestos related Lung Cancer Tissue

Paraffin embedded asbestos related lung cancer tissue of ten cases was analyzed with polymerase chain reaction single strand conformation polymorphism (PCR SSCP) to study its genetic mutation of exon 5, 7 and 8 of anti oncogene p53 by silver stain. Results showed that fragment of exon 7 or 8 of p53 gene in four cases was positive in silver staining analysis of PCR SSCP. Exon 7 or 8 fragments of p53 gene was detected positive for mutation in seven of these ten cases with autoradiographic analysis of PCR SSCP. No exon 5 fragment was found positive for mutation in these ten cases with both methods. Agreement between results of silver staining and those of autoradiographic PCR SSCP was 60%. Hence, autoradiographic method could be replaced by silver staining, a simpler and more sensitive one, in PCR SSCP to study gene mutation of asbestosrelated lung cancer.