肺癌中p53基因突变类型和可能致癌因素分析

为了研究肺癌中ｐ５３基因的作用及突变类型与不同致癌物的可能关系，用ＰＣＲ－ＳＳＣＰ核酸序列技术分析了原发非小细胞肺癌中ｐ５３基因的突变谱，结果发现１２８例中９４例发生突变，其中８６例为点突变，７例为缺失和插入突变，１例为拼接部位突变。点突变以Ｇ→Ｔ和Ｔ→Ｇ突变为主（６３．９％），Ｇ→Ａ和Ａ→Ｇ占２５．６％，这些结果提示ｐ５３基因突变与非小细胞肺癌发生有密切关系，并根据其突变特点分析了可能的环境致癌物。

Analysis of Mutation Types of p53 Gene and Possible Carcinogenic Factors in Lung Cancer

Mutational spectrum of p53 gene was analyzed by polymerase chain reaction (PCR) single strand conformational polymorphism (SSCP) and direct DNA sequencing technique in tissue specimens of primary non small cell lung cancer (NSCLC) to study the action of p53 gene, its type of mutations and its possible relation to different carcinogens. Results revealed that mutation of p53 gene was found in 94 of 128 resected specimens of NSCLC, including point mutations in 86 cases, deletion and insertion in seven and mutation of the linking site in one, and 63.9 percent of point mutations were G T and T G substitution and 25.6 percent G A and A G. It suggested that p53 gene mutation correlated closely to the occurrence of NSCLC. Possible environmental carcinogens were discussed in the paper based on the characteristics of the p53 gene mutation.