| 环氧乙烷对大鼠DNA损伤机理的研究 |
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| 引用本文： | 张龄,钟先玖.环氧乙烷对大鼠DNA损伤机理的研究[J].中华预防医学杂志,1997,31(4):199-201. |
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| 作者姓名： | 张龄 钟先玖 |
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| 作者单位： | 上海职工医学院，上海医科大学附属金山医院，复旦大学遗传学研究所 |
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| 摘 要： | 为探讨环氧乙烷（ＥｔＯ）对ＤＮＡ分子水平的损伤作用，采用血红蛋白基因特异性的单引物，对用ＥｔＯ染毒不同时间的Ｗｉｓｔａｒ雄鼠和对照个体的肝、肾、睾丸等组织的ＤＮＡ进行半随机ＰＣＲ扩增。结果显示：在吸入染毒（８００ｍｇ／ｍ３）６～９周条件下，ＥｔＯ可引起肝、肾、睾丸等组织不同程度的ＤＮＡ损伤，其中睾丸组织ＤＮＡ损伤最为严重。
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| 关 键 词： | 环氧化合物 血红蛋白类 DNA引物 |
A Study on the Mechanism of Damage to DNA in Rats by Ethylene Oxide |
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| Zhang Ling,Zhong Xianjiu,Zhang Min,et al..A Study on the Mechanism of Damage to DNA in Rats by Ethylene Oxide[J].Chinese Journal of Preventive Medicine,1997,31(4):199-201. |
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| Authors: | Zhang Ling Zhong Xianjiu Zhang Min |
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| Institution: | Zhang Ling,Zhong Xianjiu,Zhang Min,et al. Shanghai Medical College for Health Professional,Shanghai 200237 |
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| Abstract: | DNA in the liver, kidney, testes, etc. was amplified with semi random polymerase chain reaction (PCR) in Wistar male rats exposed to ethylene oxide (EtO) for various times and in control rats with hemoglobin gene specific primer to study the damage effects of EtO on DNA molecule. Results showed that inhalation of 800 mg/m 3 of EtO for six to nine weeks could cause damage to DNA in the liver, kidney and testes, etc. to various extent, with the most serious damage to DNA in the testes. |
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| Keywords: | Epoxy compounds Hemoglobins DNA primers |
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