三种尿微量蛋白检测对糖尿病早期肾损害意义的探讨

目的 :探讨尿蛋白定性阴性的糖尿病肾病 (DN)患者随机尿中三种微量蛋白检测对发现糖尿病早期肾损害的意义。方法 :尿MA .TRF和α1-MG用速率散射比浊法 ,尿Cr 采用Jaffe比色法进行测定。结果 :尿蛋白定性阴性的DN组尿MA/Cr为 ( 3 5 0 0± 2 780 )mg/mmol,TRF/Cr为 ( 0 3 3 7± 0 2 89)mg/mmol,α1-MG/Cr为 ( 3 185± 2 980 )mg/mmol。对照组尿MA/Cr为 ( 0 60 8± 0 178)mg/mmol,TRF/Cr为 ( 0 15 2± 0 0 91)mg/mmol,α1-MG/Cr为 ( 0 872±0 443 )mg/mmol。两组比较具有极显著性差异 (P <0 0 1)。结论 :尿MA、TRF及α1-MG可作为发现糖尿病早期肾损害敏感而又可靠的常规指标。

Significance of determination of urine microprotein for diagnosing early renal disease in patients with diabetes mellitus

Objective To discover early renal disease of diabetes mellitus (DM) by determining three microproteins in random urine.Methods Urine microproteins were detected by the method of immunoturbidimetry.Urinary creatinine was measured using colorimetry.Results The levels of urinary MA/Cr,TRF/Cr and α 1-MG/Cr in urinary albumin negative group were (3 500±2 780)mg/mmol,(0 337±0 289)mg/mmol,(3 185±2 980)mg/mmol respectively.The levels in the controls were (0 608±0 178)mg/mmol,(0 152±0 091)mg/mmol,and (0 872±0 443)mg/mmol respectively.There was marked difference between two groups (P<0 01).Conclusions The urinary MA,TRF and α 1-MG can be used as sensitive and reliable indexes for early renal disease of DM.