腺病毒介导的P~(53)基因对涎腺腺样囊性癌细胞系生长的影响

目的研究P53基因对涎腺腺样囊性癌细胞系生长的影响。方法以人涎腺腺样囊性癌细胞系SACC-83、SACC-LM、ACC-2、ACC-M和NACC为实验对象,将载有人野生型P53cDNA的重组腺病毒(Ad5CMV-P53)感染涎腺腺样囊性癌细胞系,观察Ad5CMV-P53对涎腺腺样囊性癌细胞生长的影响。结果5种涎腺腺样囊性癌细胞系转染Ad5CMV-P53病毒后,均可以诱导其发生凋亡,使细胞系生长受到明显的抑制。结论Ad5CMV-P53介导的野生型P53基因,可能是一种有效的治疗涎腺腺样囊性癌的辅助手段。

Effect of Adenovirus-mediated P53 Gene on Human Salivary Adenoid Cystic Carcinoma Cell Lines

Objective To study the influence of P53 gene on human salivary adenoid cystic carcinoma cell lines growth.Methods The human salivary adenoid cystic carcinoma cell lines such as SACC-83,SACC-LM,ACC-2,ACC-M and NACC were used.Recombinant cytomegalovirus-promoted adeno-viruses containing human wild-type P53 cDNA（Ad5CMV-P53） was infected into these cell lines.The growth of these cell lines were studied by MTT after that Ad5CMV-P53 virus were transfected into five cell lines.Results After these five cell lines were transfected Ad5CMV-P53 viruses,all of these cell lines appeared apoptosis and their cell growth were greatly suppressed.Conclusion Transfection of wild-type P53 gene via Ad5CMV-P53 is a potential approach to the therapy of salivary adenoid cystic carcinoma.