| HPLC法测定刺五加浓缩液中紫丁香苷的含量 |
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| 引用本文： | 孔祥臣,闫秋野,林兰波,王厚伟. HPLC法测定刺五加浓缩液中紫丁香苷的含量[J]. 中国实用医药, 2010, 5(6): 65-66 |
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| 作者姓名： | 孔祥臣 闫秋野 林兰波 王厚伟 |
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| 作者单位： | 黑龙江省农业职业技术学院,154002 |
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| 摘 要： | 目的采用高效液相色谱法测定刺五加浓缩液中紫丁香苷的含量。方法选用Agi-lentC18色谱柱,流动相：甲醇-水（20：80）;检测波长：265nm。结果紫丁香苷对照品线性范围0.1305～1.0440μg（r=1.0000）,平均回收率为99.42%,RSD=0.82%（n=6）。结论HPLC法简便,准确,重现性好。可用于刺五加浓缩质量控制。
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| 关 键 词： | 刺五加浓缩液 紫丁香苷 HPLC |
Determination of syringin in ciwujia concentrated liquid by HPLC |
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| Affiliation: | KONG Xiang-chen,YAN Qiu-ye,LIN Lan-bo,et al. (Heilongjiang agricultural college of vocational technology,Heilongjiang Jiamusi 154002,China ) |
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| Abstract: | Objective A HPLC methods was developed for the contents determination of Syringin in Ciwujia Concentrated Liquid. Methods The AgilentC18 column was used with a mobil phase was Methanol-Water（20：80）. The detection Wavelength was 265nm. Results The Linear range of reference substance of Syringin was 0. 130 5-1. 044 0 μg（r = 1. 000 0）,The average recovery rate was 99. 42% and the RSD = 0. 82% （n = 6）. Conclusion The method simple,sensitive and accurate. It can be used for quality of Ciwujia Concentrated Liquid. |
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| Keywords: | HPLC |
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