BHD基因在人肺腺癌A549细胞的表达及其对A549细胞转移能力的影响

目的 了解BHD基因在肺癌细胞中的表达部位及其在影响肺癌细胞转移、运动方面的作用. 方法 制备BHD真核表达质粒,转染肺腺癌细胞,用免疫荧光法检测Folliculin蛋白在肺癌细胞中的表达位置;筛选sRNAi片段,选取有效抑制片段转入肺癌细胞,与转入BHD质粒的肺癌细胞对比,用Transwell实验了解BHD基因对肺癌细胞运动方面的影响. 结果 转入BHD质粒后,A549细胞质内出现明显的Folliculin蛋白表达,Transwell实验显示,转入BHD质粒能抑制A549细胞的侵袭能力,而转入BHD siRNA片段的A549细胞运动迁移能力升高.结论 BHD表达产物Folliculin蛋白在肺癌细胞的胞质内表达,BHD可能是肺癌转移抑制基因.

The expression of BHD gene in human lung adenocarcinoma A549 cells and its effect of BHD gene on the metastasis of A549 cells

Objective This study was designed to investigate the BHD gene expression location in human lung cancer cells and its effect on the invasion ability of lung cancer cells. Methods The BHD vector and the small interfer RNA targeting BHD was constructed and transfected into the cell line A549. The expression of BHD was examined by immunofluorescence. Transwell test was used to detect the invasion ability of A549 cells. Results After transfected BHD vector into A549 cells, immunofluorescence showed the expression of Folliculin located in cellular plasma. The invasion ability of A549 cells decreased after transfected with BHD vector, and the invasion ability of A549 cell increased after transfected with BHD siRNA. Conclusion In the lung cancer cell line A549, Folliculin is expressed in the cytoplasm and BHD gene is a metastasis suppressor gene.