| 绒毛直接制备染色体的改良方法 |
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| 引用本文： | 黄国宁,任子骥,刘红,何舒.绒毛直接制备染色体的改良方法[J].重庆医学,1986(6). |
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| 作者姓名： | 黄国宁 任子骥 刘红 何舒 |
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| 作者单位： | 重庆市妇幼保健院优生研究室
(黄国宁，任子骥，刘红)，重庆市妇幼保健院优生研究室(何舒) |
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| 摘 要： | 本文针对绒毛直接制备染色体中存在分裂和质量不稳定的情况，而增加固定液作用时间。将低渗后的绒毛细胞换固定液后置冰箱（±4℃）过夜，并在60％冰乙酸解离绒毛细胞后再换固定液作用3小时或置冰箱过夜。结果所获得染色体形态、分散较以前改进。并可获得较高质量的分裂相。21例计数结果每例可分析的染色体占分裂相数的40.93％。
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| 关 键 词： | 绒毛细胞 染色体 固定液 |
IMPROVEMENT OF THE DIRECT CHROMOSOMEPREPARATION FROM CHORIONIC VILLI |
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| Huang Guoling,et alChongqing Women and Children Health Hospital,Chongqing.IMPROVEMENT OF THE DIRECT CHROMOSOMEPREPARATION FROM CHORIONIC VILLI[J].Chongqing Medical Journal,1986(6). |
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| Authors: | Huang Guoling Chongqing Women and Children Health Hospital Chongqing |
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| Abstract: | The direct chromosome preparation from chorionic villi was a simple and rapid method, but the unstable condition of chromosome influenced karyotyping. In order to solve the problem, we improved the preparation method by prolongation of the action period of fixative. The result showed good chromosome shape, nice separability and high quality metaphases. |
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| Keywords: | chorionic cell chromosome fixative |
| 本文献已被 CNKI 等数据库收录！ |
