TFPI-2对胰腺癌细胞增殖及血管新生的抑制作用

目的 探讨胰腺癌中组织因子途径抑制物2(TFPI-2)的表达及作用。方法 采用免疫组织化学方法检测41例胰腺癌组织中TFPI-2，Ki67和VEGF的表达及CD34单克隆抗体标记的微血管密度（MVD）。结果 Ⅰ，Ⅱ期胰腺癌TFPI-2阳性表达率（17/26）高于Ⅲ，Ⅳ期（2/15），高分化癌（12/19）高于中、低分化（7/22）（均P<0.05）；Ⅰ，Ⅱ期癌细胞增殖指数（PI）（24.45±6.19）低于Ⅲ，Ⅳ期癌（30.84±6.70），高分化癌（24.42±6.29）低于中、低分化癌（28.84±7.12），（均为P< 0.05）；Ⅰ，Ⅱ期癌VEGF的阳性表达率（16/26）低于Ⅲ，Ⅳ期癌（14/15），高分化癌（11/19）低于中、低分化癌（19/22）（均P<0.05）；Ⅰ，Ⅱ期癌MVD值（26.92±1.06）低于Ⅲ，Ⅳ期癌（28.47±2.23），高分化癌（19.00±2.58）低于中、低分化癌（16.68±1.79）（均P< 0.05）。胰腺癌组织中TFPI-2的表达与Ki67和VEGF的表达呈负相关（r=－0.62，P<0.05；r=－0.54，P<0.05），MVD与TFPI-2表达亦有相关。结论 TFPI-2表达能够抑制胰腺癌细胞增殖，并通过下调VEGF的表达抑制肿瘤新生血管的形成，这可能是其抑制胰腺癌的生长、转移的重要机制。

Inhibitory effect of tissue factor pathway inhibitor 2 on cell proliferation of pancreatic carcinoma cells and tumor angiogenesis

Abstract:Objective To explore the expression and action of tissue factor pathway inhibitor 2 (TFPI-2) in pancreatic carcinoma (PC).Methods Tissue specimens from 41 pancreatic cancers were used to detect the expression of TFPI-2, Ki67, VEGF and MVD tagged with CD34 by immunohistochemistry.Results The positive expression of TFPI-2 in stage Ⅰand Ⅱ PC (17/26) was higher than that in stage Ⅲ and Ⅳ (2/15) PC. The positive expression of TFPI-2 in welldifferentiated PC (12/19) was also higher than that in moderate and poorlydifferentiated PC (7/22)(P< 0.05). The cell proliferation index in stage Ⅰ,Ⅱ(24.45±6.19) and welldifferentiated PC (24.42±6.29) was lower than that in stage Ⅲ, Ⅳ (30.84±6.70) and moderate, and poorlydifferentiated PC (28.84±7.12)(P< 0.05). The positive expression of VEGF in stage ⅠandⅡ PC (16/26) was higher than that in stage Ⅲ and Ⅳ (14/15). The positive expression of VEGF in welldifferentiated PC (11/19) was also higher than that in moderate and poorlydifferentiated PC (19/22)(P< 0.05). The value of MVD in stage Ⅰ,Ⅱ(26.92±1.06) and welldifferentiated PC (19.00±2.58) was lower than that in stage of Ⅲ, Ⅳ (28.47±2.23)  and moderate and poorlydifferentiated PC (16.68±1.79), (P< 0.05). The expression of TFPI-2, Ki67 and VEGF was negatively correlated. The MVD was related to the expression of TFPI-2.Conclusions TFPI-2 can inhibit cell proliferation of PC, and by reducing the expression of VEGF can inhibit the angiogenesis of new vessels in PC, which may play an important part in inhibitory effect on invasion and metastasis of PC.