Stability of lactate dehydrogenase at different storage temperatures |
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| Authors: | E Jacobs P J Hissin W Propper L Mayer L Sarkozi |
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| Institution: | 1. Graduate Program in Cardiovascular Sciences, Fluminense Federal University (UFF), Niterói, Rio de Janeiro, Brazil;2. Graduate Program in Medical Sciences, Fluminense Federal University (UFF), Niterói, Rio de Janeiro, Brazil;3. Graduate Program in Nutrition Sciences, Fluminense Federal University (UFF), Niterói, Rio de Janeiro, Brazil;4. Medicine Faculty, Fluminense Federal University (UFF), Niterói, Rio de Janeiro, Brazil;1. Institute of Surgical Pathology, University Hospital Zurich, Schmelzbergstrasse 12, 8091 Zurich, Switzerland;2. Department of Computer Science, ETH Zurich, Universitaetstr 6, 8092 Zurich, Switzerland;3. Institute of Pathology, Cantonal Hospital Winterthur, Brauerstrasse 15, 8401 Winterthur, Switzerland;4. Department of Urology, University Hospital Zurich, Rämistrasse 100, 8091 Zurich, Switzerland;5. Department of Plastic, Reconstructive and Aesthetic Surgery, University Hospital Basel, Spitalstrasse 21, 4031 Basel, Switzerland;6. Institute of Pathology, University Hospital Bonn, Sigmund-Freud-Strasse 25, 53127 Bonn, Germany;7. Department of Urology, City Hospital Triemli, Birmensdorferstrasse 497, 8063 Zurich, Switzerland;1. Applied Chemistry, Chemical and Biological Engineering, Chalmers University of Technology, S-412 96 Göteborg, Sweden;2. Department of Chemistry and Molecular Biology, University of Gothenburg, S-412 96 Göteborg, Sweden;3. Department of Energy and Resources, University College London, 220 Victoria Square, Adelaide, SA 5000, Australia;1. Department of Medicine, Marienhausklinik St. Josef Kohlhof, Klinikweg 1-5, 66539 Neunkirchen, Germany;2. Department of Medicine II, Saarland University Medical Center, Saarland University, Homburg, Germany |
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| Abstract: | The effect of storing human serum, cord blood serum or heparinized plasma at 25 degrees C, 4 degrees C & -20 degrees C on the activity and isoenzyme distribution of lactate dehydrogenase (LD) was studied. Cellulose acetate and agarose electrophoresis, as well as an immunochemical inhibition technique, were used for isoenzyme quantification. In contrast to previous reports, cryo-instability was found only in specimens stored at 4 degrees C. Serum specimens stored at 25 degrees C and -20 degrees C retained 74% and 87% of total activity after 45 days of storage. LD-1 was stable at all three temperatures, with a maximum loss of 10%. LD-2, LD-3, LD-4, & LD-5 were most labile at 4 degrees C. Specimens that are to be analyzed for total LD or LD isoenzymes should be stored frozen or, if necessary, at room temperature, but not in a refrigerator. Thus, separate storage of specimens for cardiac isoenzymes (LD & creatine kinase) is not necessary. This may eliminate a possible source of falsely elevated LD-1/LD-2 ratios, as well as reducing the labor factor and the corresponding cost of cardiac isoenzyme determinations. |
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