槲皮素对TNF-α损伤的血管内皮细胞的保护作用

目的:用TNF-α诱导血管内皮细胞(ECV-304)损伤,观察槲皮素对ECV-304的保护作用.方法:采用MTT法观察槲皮素对ECV-304活性的影响:硫代巴比妥酸法测定细胞内脂质过氧化产物丙二醛(MDA)含量;用比色法测定细胞培养液中超氧化物歧化酶(SOD)和一氧化氮(NO)含量;免疫细胞化学法观察内皮细胞核转录因子-κB(NF-κB)的表达.结果:TNF-α对ECV-304具有损伤作用,槲皮素能显著抑制TNF-α诱导ECV-304内NO和MDA释放和NF-κB的表达;并能提高TNF-α诱导ECV-304内SOD活性.结论:槲皮素对TNF-α诱导ECV-304损伤具有保护作用,其机制可能与其减少NO释放和抗氧化作用有关,而抗氧化作用主要是通过调节NF-κB的表达.

Protection of Vascular Endothelial Cells from TNF-α Induced Injury by Quercetin

OBJECTIVE: To investigate the effects of quercetin (Que) on the TNF-alpha injured human umbilical vein endothelial cell line (ECV-304). METHODS: The experiments were performed in culture of TNF-alpha-induced ECV-304 in vitro. Cell viability was assessed with MTT assay. Lipid peroxidation of ECV-304 was monitored as malonaldehyde (MDA) with a fluorometric assay. NO and SOD of ECV-304 were monitored with colorimetry. The expression of NF-kappaB was detected with immunocytochemistry. RESULTS: TNF-alpha inhibited the ECV-304 prolifteration. Preincubation of ECV-304 with Que for 24h before TNF-alpha exposure increased the cell viability. Que significantly decreased the level of NO and MDA and the expression of NF-kappaB, and increased the activity of SOD in TNF-alpha-induced ECV-304. CONCLUSION: These results demonstrate that Que can produce the protective action on TNF-alpha-induced cultured ECV-304 and its mechanism of action may be related to the decrease of NO, antioxidant effect of lipids. However, antioxidant effect might be exerted through NF-kappaB activation pathway.