nm23H1和S100A4mRNA表达与胃癌细胞体外侵袭力的关系

目的探讨S100A4、nm23H1基因mRNA表达与胃癌细胞体外侵袭力的关系.方法采用Boyden小室法测定4种胃癌细胞系MKN1、MKN45、GT3TKB、BGC823的体外侵袭力,RT-PCR检测4种细胞系的S100A4和nm23H1mRNA表达水平.结果胃癌细胞体外侵袭力高低的顺序依次为MKN45最高,BGC823、MKN1次之(二者无显著差异),GT3TKB最低(P<0.001).nm23H1、S100A4基因mRNA各自表达与胃癌细胞体外侵袭力无明显关系.nm23H1/S100A4mRNA相关表达水平的顺序依次为GT3TKB最高,BGC823和MKN1次之(二者无显著差异,P>0.05)、MKN45最低(P<0.001),其相关表达与胃癌细胞体外侵袭力呈反比关系.结论nm23H1/S100A4mRNA的相关表达在评价胃癌细胞体外侵袭力方面具有重要价值.

The Correlation of nm23H1 and S100A4 mRNA Expression With in Vitro Invasive Capacity of Gastric Cancer Cells

Objective: Our aim was to evaluate the correlation of the mRNA expression of S100A4 and nm23H1 gene with the in vitro invasive capacity of gastric cancer cells.Methods:In vitro invasive capacity of gastric cancer cells was determined by using Boyden chamber method . The mRNA expressions of S100A4 and nm23H1 gene were measured in gastric cancer cell lines by using RT-PCR . Results:The order of the invasive capacity of gastric cancer cells were: MKN45 was the highest,BGC823 and MKN1 were the second (there were no significant difference between them, P>0.05), GT3TKB was the lowest(P< 0.001).The mRNA expressions of the two genes were not related to the invasive capacity of gastric cancer cells. The order of the relative expression of nm23H1/S100A4 mRNA were: GT3TKB the highest;BGC823 and MKN1 the second (there were no significant difference between them,P>0.05), MKN45 was the lowest(P< 0.001). The relative expression of nm23H1/S100A4 mRNA was negatively correlated with the in vitro invasive capacity of gastric cancer cells. Conclusion:The relative expression of nm23H1/S100A4 mRNA has an important value in evaluating invasive capacity of gastric cancer cell lines.