Survivin基因在人胃癌及癌前病变中表达的研究

目的探讨凋亡抑制基因survivin在胃癌及癌前病变组织中的表达。方法应用RT—PCR技术研究survivin mRNA在46例胃癌组织及相应正常组织中的表达，ELISA技术定量检测上述标本中survivin蛋白表达，免疫组织化学染色（IHC）Envision法定性检测46例胃癌标本、114例胃炎及癌前病变组织survivin蛋白表达。结果46例胃癌组织的survivin mRNA阳性表达率为32．6％,46例相应正常组织为10．87％。两组差异有统计学意义（P〈O．05o胃癌组的survivin蛋白表达浓度（75．27—358．44pg/ml）显著高于正常组（0—161．23pg／ml）（P〈0．05）。Survivin蛋白阳性表达率在浅表性胃炎（30％）、萎缩性胃炎（14．7％）、胃黏膜肠化组织（30％）差异均无统计学意义（均P〉0．05）。与不典型增生（60％）、胃癌组织（46．5％）差异均有统计学意义（均P〈0．05）。胃癌组织survivin mRNA、蛋白表达阳性率及浓度在不同性别、肿瘤长径、发生部位、大体类型、组织类型、分化程度、有无脉管侵犯、浸润深度、有无淋巴结转移、有无远处转移、TNM分期间差异均无统计学意义（均P〉0．05）。结论Survivin在胃癌、胃黏膜不典型增生中的表达高于正常组织。提示与胃癌发生有关；与临床病理特征无关，提示survivin可能在肿瘤的形成阶段起作用，与胃癌发展侵袭无关。

The expression of survivin in human gastric carcinoma and precancerous lesions

Objective To investigate the expressions of survivin in gastric carcinomas and precancerous lesions. Methods Survivin mRNA expression was quantitatively determined by RT-PCR, and the protein contents were quantitatively determined by ELISA in 46 specimens of gastric carcinomas and 46 specimens of normal gastric tissues. Survivin protein expressions were also examined immunohistochemically in 46 specimens of gastric carcinoma tissues and 114 specimens of gastritis and precancerous lesion tissues. Results Survivin mRNA was expressed in 32.6% of gastric carcinomas (15/46), while it was expressed in 10.8% of neighboring normal tissues (P<0.05). The survivin protein contents in gastric carcinoma were significantly higher than those in normal tissue(P<0.05). There were no significant differences in survivin protein expression among superficial gastritis, atrophic gastritis, and intestinal metaplasia, but significant differences were detected among superficial gastritis, atrophic gastritis, intestinal metaplasia, gastric dysplasia, gastric carcinoma (P<0.05). The survivin mRNA expression, protein expression and the protein contents were not associated to gender of patient, the size, depth, location, gross type, differentiation, vein invasion, lymph nodes metastasis, distant metastasis, TNM staging of tumor (all P>0.05). Conclusion These data suggest that survivin may be involved in the inhibition of apoptosis in gastric carcinomas, and may be relevant to the genesis of gastric carcinoma, but irrelevant to the development and invasion of tumors.