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Hippocampus‐prefrontal cortex inputs modulate spatial learning and memory in a mouse model of sepsis induced by cecal ligation puncture
Authors:Cheng‐  Long Ge,Wei Chen,Li‐  Na Zhang,Yu‐  Hang Ai,Yu Zou,Qian‐  Yi Peng
Affiliation:1. Department of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha Hunan Province, China ; 2. National Clinical Research Center for Geriatric Disorders, Changsha Hunan Province, China ; 3. Hunan Provincial Clinical Research Center for Critical Care Medicine, Changsha Hunan Province, China ; 4. Department of Anesthesia, Xiangya Hospital, Central South University, Changsha Hunan Province, China
Abstract:AimsSepsis‐associated encephalopathy (SAE) often leads to cognitive impairments. However, the pathophysiology of SAE is complex and unclear. Here, we investigated the role of hippocampus (HPC)‐prefrontal cortex (PFC) in cognitive dysfunction in sepsis induced by cecal ligation puncture (CLP) in mice.MethodsThe neural projections from the HPC to PFC were first identified via retrograde tracing and viral expression. Chemogenetic activation of the HPC‐PFC pathway was shown via immunofluorescent staining of c‐Fos‐positive neurons in PFC. Morris Water Maze (MWM) and Barnes maze (BM) were used to evaluate cognitive function. Western blotting analysis was used to determine the expression of glutamate receptors and related molecules in PFC and HPC.ResultsChemogenetic activation of the HPC‐PFC pathway enhanced cognitive dysfunction in CLP‐induced septic mice. Glutamate receptors mediated the effects of HPC‐PFC pathway activation in CLP mice. The activation of the HPC‐PFC pathway resulted in significantly increased levels of NMDAR, AMPAR, and downstream signaling molecules including CaMKIIa, pCREB, and BDNF in PFC. However, inhibition of glutamate receptors using 2,3‐dihydroxy‐6‐nitro‐7‐sulphamoyl‐benzo (F)quinoxaline (NBQX), which is an α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid receptor (AMPAR inhibitor), or D‐2‐amino‐5‐phosphonopentanoate (D‐AP5), which is an NMDA receptor antagonist abolished this increase.ConclusionOur study reveals the important role of the HPC‐PFC pathway in improving cognitive dysfunction in a mouse model of CLP sepsis and provides a novel pathogenetic mechanism for SAE.
Keywords:cecal ligation puncture, cognitive dysfunction, hippocampus, prefrontal cortex, sepsis‐  associated encephalopathy
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