| 球形幽门螺杆菌致细胞空泡变性毒力研究 |
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| 引用本文： | 余菲菲,朱苹,史碧山,苏东辉,强华,陈月秀,郑秀芬.球形幽门螺杆菌致细胞空泡变性毒力研究[J].中国人兽共患病杂志,2000,16(2):50-53. |
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| 作者姓名： | 余菲菲 朱苹 史碧山 苏东辉 强华 陈月秀 郑秀芬 |
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| 作者单位： | [1]福建医科大学微生物学教研室 [2]福建医科大学附属第一医院消化内科 |
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| 基金项目： | 本课题受福建省自然基金资助(基金编号95A003) |
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| 摘 要： | 目的 揭示球菌幽门螺杆菌致细胞空泡变性毒力的变异情况。方法 采用延期培养和加亚抑菌浓度抗生素，对细胞毒相关蛋白基因阳性（ｃａｇＡ＾＋），和细胞空泡毒素基因阳性（ｖａｃＡ＾＋）的高毒株幽门螺杆菌（Ｈｐ）进行球形诱变，进而检测该Ｈｐ至Ｈｅｌａ细胞空泡变性的毒力，并用ＳＤＳ－ＰＡＧＥ、ＰＣＲ及ＰＣＲ－ＳＳＣＰ技术，分析球形Ｈｐ蛋白产物及决定细胞空泡变性的ｃａｇＡ和ｖａｃＡ基因的变异情况。结果与结论 球形
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| 关 键 词： | 球形幽门螺杆菌 细胞空泡毒素 毒力 基因突变 |
| 文章编号： | 1002-2694(2000)02-0050-04 |
STUDY ON THE VACUOLATING CYTOTOXICITY OF COCCOID HELICOBACTER PYLORI |
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| SHE Feifei, ZHU Ping, SHI Bishan, SU Doghui,et al..STUDY ON THE VACUOLATING CYTOTOXICITY OF COCCOID HELICOBACTER PYLORI[J].Chinese Journal of Zoonoses,2000,16(2):50-53. |
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| Authors: | SHE Feifei ZHU Ping SHI Bishan SU Doghui |
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| Institution: | SHE Feifei, ZHU Ping, SHI Bishan, SU Doghui, et al. |
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| Abstract: | Aim To reval the virulence variation related to vacuolating cytotoxicity of coccoid Helicobacter pylori.Methods The coccoid Helicobacter pylri, which was transformed from the bacterium with cagA gene and vacA gene by means of prolonged and antibiotics with subinhibitory conentaion, was detected the vacuolating cytotxin to Hela calls, analysed the differences of protein between the verity strain and its prototype by using SDS-PAGE, studied mutation of the vacA gene and cagA gene by means of PCR and PCR-SSCP Results and Conclusions.The vacuolating cytotoxicity,the proteins proteins with molecular weights above 74KD, especially 125KD, were decreased, no deletion was found in 259bp amplification fragments from vacA gene and 349bp amplification fragments from cagA gene but point mutation was existed in vacA gene of coccoid Hp. |
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| Keywords: | Coccoid Helicobacter pylori Vacuolating cytotoxin |
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