胃癌组织COX-2的表达及其与细胞增殖和凋亡的关系

目的 :探讨环氧化酶 - 2 (cyclooxygenase - 2 ,COX - 2 )在胃癌组织中的表达与癌细胞增殖和凋亡的关系。 方法 :采用免疫组织化学的方法检测 4 3例胃癌患者COX - 2和增殖细胞核抗原 (PCNA)的表达 ,用TUNEL法检测原位细胞凋亡的情况。分别计算细胞增殖指数 (MI)和凋亡指数 (AI)。结果 :胃癌组织COX - 2表达的阳性率为 6 0 .4 7% ,明显高于对照组 (P <0 .0 1)。COX - 2的高表达与胃癌淋巴结的转移和临床分期有关 ,与肿瘤大小、浸润深度、分化程度均无关。COX - 2阳性胃癌组中MI高于COX - 2阴性胃癌组 ,而AI却低于COX - 2阴性胃癌组 (P均 <0 .0 1)。结论 :COX - 2的过度表达可促进胃癌细胞增殖、抑制细胞凋亡 ,在胃癌的发生、发展过程中起着重要的作用。

The expression of COX- 2 and its relationship to the apoptosis and proliferation in gastric cancer

Objective:To investigate the expression level of COX-2 and their effects on cell proliferation activity and apoptosis degree in gastric cancinoma.Methods:The expression of COX-2,proliferating cell nuclear antigen(PCNA) and apoptosis in 43 cases of gastric cancer were studied by immunohistochemistry and TUNEL methods,and the proliferation index (PI) and apoptotic index(AI) were defined respectively.Results:The positive expression rate of COX-2 was 60.47% in gastric carcinoma tissue.It was significantly higher than that in the control group.Overexpression of COX-2 in gastric carcinoma was related to its lymph node metastasis and clinical stage,but it did not correlate with the size of tumor,depth of the invasion and the differentiation degree( P >0.05).In the group with COX-2 positive expression AI was higher than that of the group with COX-2 negative expression,whereas AI was higher in the latter group than in the former ( P <0.01).Conclusion:The expression level of COX-2 may have a close correlation with cell proliferation and apoptosis and they may participate in oncogenesis and progression of gastric carcinoma.