去纤酶无直接溶栓作用

在以贫含血小板血浆为媒介的体外溶栓模型上,尿激酶(5×10~5～5×10~6IU·L~(-1))作用30min使人造混合血栓的重量呈剂量依赖性的降低。剂量为2×10~6IU·L~(-1)时,作用达峰值。相反,去纤酶(6.25～25IU·L~(-1))使血栓重量呈剂量相关性的增加。用大剂量凝血酶(1×10~5IU·L~(-1))消耗媒介中的纤维蛋白原后,去纤酶不再增加血栓重量,与生理盐水相比,亦未见有明显的降低血栓重量的作用。本结果提示去纤酶无直接溶栓作用。

Defibrase shows no direct thrombolytic actions

In the in vitro model of thrombolysis designed by ourselves, urokinase (500 000~ 5 000 000IU · L-1) reduced the weight of artificial thrombi in a way of dose-dependence. At the level of 2 000 000 IU · L-1, the action reached the peak. On the contrary, defibrase (6. 25~25 IU · L-1) dose-relatedly increased the thrombus weight. After defibrinogenation in platelet-poor plasma by addition of porcine thrombin (100 000 IU · L-1) , defibrase no longer increased the thrombus weight, whereas in comparison with saline, defibrase still showed no apprciable reduction in thrombus weight. The findings hinted that defibrase may have no direct thrombolytic property.