电针对醋酸氢化可的松模型大鼠生殖功能影响的实验研究

目的:观察电针对雄性醋酸氢化可的松模型大鼠生殖功能的影响.方法:将36例SD雄性大鼠随机分为3组,正常组(n=6)不予任何处理;模型组(n=15)制备大鼠氢化可的松模型后不予任何处理;电针组(n=15)制备大鼠氢化可的松模型后给予电针治疗.用放射免疫分析法(Radioimmunoassay,RIA)测定血黄体生成素(Luteinizing Hormone,LH)和睾酮(Testosterone,T)含量,组织切片HE染色进行睾丸组织学观察.结果:动物造模后血T明显下降,与造模前比较,P<0.05;LH升高,但与造模前无统计学差异.睾丸生精细胞分层数显著降低.治疗后,电针组大鼠血LH水平明显高于模型组(P<0.05);血T水平虽有升高趋势,但差异无统计学意义.睾丸生精细胞分层数增加明显(P<0.05).结论:电针可能通过升高体内LH和T水平,调整模型大鼠的生殖功能.

Experimental research of electroacupuncture on the reproductive function of hydrocortisone acetate modeling rats

Objective： To observe the impact of electroacupuncture on the reproductive function of hydrocortisone acetate modeling rats. Methods： Thirty-six male SD rats were randomly allocated into three groups. The rats in the control group （n=6） underwent no treatment. The rats in the model group （n=15） were induced by hydrocortisone and not treated. The rats in the electroacupuncture group （n=15） were induced by hydrocortisone and treated with electroacupuncture. The luteinizing hormone （LH） and the testosterone （T） were tested with radioimmunoassay, while the hematoxylin-eosin （HE） staining was used for histological observation. Results： Compared with the pre-modeling, the serum T decreased significantly after the modeling （P0.05）, and the LH increased without significant difference. The delamination of spermatids reduced dramatically. After the treatments, the serum LH of the rats in the electroacupuncture group were significantly higher than the rats in the model group （P0.05） but the serum T increased without significant difference. The delamination of spermatids increased notablely （P0.05）. Conclusion： Electroacupuncture may regulate the reproductive function of the rats by improving the level of LH and T concentration.