Rho／Rho激酶在哮喘小鼠气道的表达及其对气道重塑的影响

目的研究Rho／Rho激酶（ROCK）信号通路在哮喘小鼠气道中的表达及其对气道重塑的作用。方法清洁级BALB／c雄性小鼠39只，随机分为对照组、哮喘组、Y-27632干预组、地塞米松干预组，每组10只（其中对照组9只）。建立哮喘小鼠模型，并应用ROCKⅡ特异性拮抗剂Y-27632进行干预，光镜观察肺组织结构，Image—proplus图像分析软件测量支气管壁厚度（Wat）和平滑肌厚度（Wam），免疫组化法测定肺组织ROCKⅡ蛋白含量，RT—PCR法测定RhoA、ROCKⅡ mRNA含量。结果（1）哮喘组小鼠RhoA、ROCKⅡ表达明显增高，应用ROCKⅡ特异性拮抗剂Y-27632能够下调RhoA、ROCKⅡ的表达。（2）Y-27632干预组小鼠Wat和Wam均明显低于哮喘组小鼠。结论哮喘小鼠肺组织RhoA及ROCKⅡ表达增多，ROCKll受体拮抗剂Y-27632能够下调RhoA、ROCKⅡ的表达，明显抑制哮喘小鼠Wat和Wam。

Expression of Rho/ROCK in asthmatic mice and its effect on airway remodeling

Objective To investigate the expression of Rho/ROCK in asthmatic mice and its effect on airway remodeling. Methods Thirty-nine male BALB/c mice were randomly divided into 4 groups： the normal group （n=9）, the asthma model group （n=10）, the Y-27632-treated group （n= 10） and the dexamethasone-treated group （n= 10）. The asthma model was established by intraperitoneal injection of 0.1% OVA. The pathological changes of lung tissue were observed. The thickness of airway smooth muscle and bronchial wall in lung tissue were measured. ROCKⅡ protein, RhoA and ROCKⅡ mRNA were detected by immunohistochemistry and RT-PCR respectively. Results The expression of RhoA and RQCKⅡ in asthma group was significantly higher than those in normal group and treated groups, and Y-27632 decreased the expression of RhoA and ROCKⅡ（P 〈 0.05）. Compared with asthma group, the brochial wall thickness and the smooth muscle thickness of Y-27632- treated group were significantly decreased （P〈 0.05）. Conclusion RhoA and ROCKⅡ are highly expressed in sensitized mouse, Y-27632 can downregulate their expression and inhibit the thickening of brochial wall and smooth muscles.