| 糖基化终末产物对人脐静脉内皮细胞凋亡及其受体表达的影响 |
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| 引用本文： | 郑超,文格波,曹仁贤,刘江华,黄雌友. 糖基化终末产物对人脐静脉内皮细胞凋亡及其受体表达的影响[J]. 中华内分泌代谢杂志, 2003, 19(2): 115-118 |
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| 作者姓名： | 郑超 文格波 曹仁贤 刘江华 黄雌友 |
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| 作者单位： | 1. 温州医学院附属第二医院内科,325007
2. 421001,衡阳,南华大学附属第一医院临床医学研究所 |
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| 基金项目： | 湖南省教委基金资助项目 (97B1 0 3)，湖南省卫生厅资助(9758) |
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| 摘 要： | 目的 研究糖基化终末产物(AGEs)对人脐静脉内皮细胞(HUVEC)凋亡及其受体(RAGE)表达的影响。方法 采用流式细胞仪技术，荧光显微镜，电镜观察AGEs对培养的HUVEC凋亡的影响，同时用RT-PCR方法检测RAGE的mRNA的表达。结果 AGEs加入细胞培养中，可明显诱导HUVEC凋亡，并呈剂量依赖效应关系。在发生凋亡的同时有RAGE mRNA表达的增强。结论 AGEs对HUVEC有诱导凋亡的作用，该作用可能通过RAGE介导。
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| 关 键 词： | 糖基化终末产物 人脐静脉内皮细胞 细胞凋亡 受体 流式细胞仪 检测 基因表达 |
| 修稿时间： | 2001-09-21 |
Effect of advanced glycosylation end products (AGEs) on apoptosis of human umbilical vein endothelial cells and mRNA expression of AGEs receptor |
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| ZHENG Chao,WEN Ge-bo,CAO Ren-xian,LIU Jiang-hua,Huang Ci-you. Clinical Medicine Research Institute,The First Hospital Affiliated to Nanhua University,Hengyang. Effect of advanced glycosylation end products (AGEs) on apoptosis of human umbilical vein endothelial cells and mRNA expression of AGEs receptor[J]. Chinese Journal of Endocrinology and Metabolism, 2003, 19(2): 115-118 |
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| Authors: | ZHENG Chao WEN Ge-bo CAO Ren-xian LIU Jiang-hua Huang Ci-you. Clinical Medicine Research Institute The First Hospital Affiliated to Nanhua University Hengyang |
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| Affiliation: | ZHENG Chao,WEN Ge-bo,CAO Ren-xian,LIU Jiang-hua,Huang Ci-you. Clinical Medicine Research Institute,The First Hospital Affiliated to Nanhua University,Hengyang 421001 |
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| Abstract: | Objective To explore the mechanism of advanced glycosylation end products (AGEs) in damaging human umbilical venous endothelial cells (HUVEC) and affecting the expression of receptor of AGEs (RAGE). Methods The effect of AGEs on apoptosis of cultured HUVEC was studied by flow cytometry technique, fluoresence microscope and electron microscope. The expression of RAGE mRNA was also assayed by RT-PCR. Results The ratio of apoptosis was 6.5% in control, but increased to 12.8%, 31.2%, 93.4% when treated with 10, 50, 100 mg/L of AGEs. The effects were dose-dependent. RAGE mRNA expression was much higher in apoptosis cells than that in normal cells. Conclusion AGEs induce apoptosis in HUVEC, which might be mediated by RAGE. |
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| Keywords: | Glycosylation end products advanced Endothelium vascular Apoptosis |
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