| 脐血淋巴因子激活的杀伤细胞杀瘤活性的研究 |
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| 引用本文： | 何跃东,潘小玲,刘珊玲,彭芝兰.脐血淋巴因子激活的杀伤细胞杀瘤活性的研究[J].中国输血杂志,2007,20(6):491-493. |
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| 作者姓名： | 何跃东 潘小玲 刘珊玲 彭芝兰 |
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| 作者单位： | 四川大学华西第二医院,四川成都,610041 |
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| 摘 要： | 目的研究脐血来源的淋巴因子激活的杀伤细胞用于过继免疫治疗的可行性。方法从脐血中分离出单个核细胞,用IL-2刺激扩增;用FCM检测表型变化,MTT比色法检测其对SKOV6及K562细胞的杀伤活性,并与用同样方法所得外周血来源的淋巴因子激活的杀伤细胞相对照。结果脐血来源的淋巴因子激活的杀伤细胞有明显的抑瘤作用。结论脐血可作为过继免疫治疗中效应细胞来源用于癌症的治疗。
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| 关 键 词： | 脐血 杀伤细胞/淋巴因子激活 过继免疫治疗 |
| 文章编号： | 1004-549X（2007）06-0491-03 |
| 收稿时间： | 2006-09-28 |
| 修稿时间： | 2007-01-25 |
The lethal activity of lymphokine activated killer cells from umbilical blood on some human cancer cells |
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| HE Yuedong. PAN Xiaoling, LIU Shanling,et al..The lethal activity of lymphokine activated killer cells from umbilical blood on some human cancer cells[J].Chinese Journal of Blood Transfusion,2007,20(6):491-493. |
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| Authors: | HE Yuedong PAN Xiaoling LIU Shanling |
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| Institution: | The Second Hospital of Sichuan University, Chengdu, Sichuan 610041, China |
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| Abstract: | Objective To investigate the feasibility of lymphokine activated killer(LAK) cells induced from cord blood used as adoptive cellular immunotherapy for human cancer.Methods Mononuclear cells were separated from umbilical blood(UBMC) by Ficoll,and stimulated by IL-2.The phenotypes(CD3/ CD4/ CD8) of the mononuclear cells were assayed by Flow cytometry,and their lethal activity on K562 or SKOV6 assayed by MTT colometric.The peripheral blood mononuclear cells were used as the control.Results The in vitro anti-tumor effect of LAK from cord blood was significant.Conclusion LAK from cord blood can be a source of adoptive cellular immunotherapy in the treatment of human cancer. |
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| Keywords: | Cord blood Lymphokine activated killer Adoptive cellular immunotherapy |
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