| 细菌耐利福定与RNA多聚酶的关系 |
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| 引用本文： | 张欣凤,蒋天蓉,李显志,王浴生.细菌耐利福定与RNA多聚酶的关系[J].四川大学学报(医学版),1991(1). |
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| 作者姓名： | 张欣凤 蒋天蓉 李显志 王浴生 |
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| 作者单位： | 基础医学院药理学教研室
(张欣凤，蒋天蓉，李显志)，基础医学院药理学教研室(王浴生) |
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| 基金项目： | 国家自然科学基金，本校青年科学基金 |
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| 摘 要： | 以药物梯度平皿筛选得到金黄色葡萄球菌NCTC6571、大肠杆菌NCTC10418及普通变形杆菌1065的耐利福定菌株。利福定能明显抑制~3H-尿苷掺入敏感菌株,而对耐药菌株的RNA合成无明显影响;利福定与利福平对标准RNA多聚酶活性的抑制随着药物剂量的增加而加强;提取及纯化RNA多聚酶,利福定能明显抑制敏感菌的酶活性,而对金黄色葡萄球菌NCTC6571与大肠杆菌NCTC10418耐药菌株的酶活性无明显影响。
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| 关 键 词： | 利福定 耐药性 RNA多聚酶 ~3H-尿苷 ~3H-三磷酸腺苷 |
Relationship between the Bacterial Resistance Induced by Rifandin and the RNA Polymerase |
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| Zhang Xinfeng,Jiang Tianrong,Li Xianzhi,Wang Yusheng.Relationship between the Bacterial Resistance Induced by Rifandin and the RNA Polymerase[J].Journal of West China University of Medical Sciences,1991(1). |
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| Authors: | Zhang Xinfeng Jiang Tianrong Li Xianzhi Wang Yusheng |
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| Institution: | Zhang Xinfeng,Jiang Tianrong,Li Xianzhi,Wang Yusheng Department of Pharmacology,School of Basic Medical Sciences |
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| Abstract: | The results of our experi-ment in vitro showed that the activity ofstandard RNA polymerase decreased whilethe concentration of RFD and RFP increas-ed, indicating that the target site of actionof RFD was similar to that of RFP on RNApolymerase. Incoporation of ~3H]UR intothe RNA of sensitive cells of S. sureus and RFD did not affect the resistant strains.In purified DNA-dependent RNA poly-merase of sensitive and resistant strains,the enzyme activity of sensitive E. coli aS. aureus strains was inhibited by RFD,but RFD did not inhibit that of the resis-tant strains(except the P. vulgaris). Itcan be concluded that a decreased suscep-tibility of RNA polymerase to RFD as wellenvelope may induce bacterial resistanceto the drug. |
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| Keywords: | Rifandin Resistance RNA polymerase [~3H]UR [~3H]AIP |
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