| 改良型序列特异性引物PCR用于脂联素基因单核苷酸多态性的检测 |
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| 引用本文： | 王延蛟,孙丽丽,陈红,焦谊,刘晓宇,张成,艾克拜尔·艾力,关亚群.改良型序列特异性引物PCR用于脂联素基因单核苷酸多态性的检测[J].地方病通报,2010(2):4-6. |
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| 作者姓名： | 王延蛟 孙丽丽 陈红 焦谊 刘晓宇 张成 艾克拜尔·艾力 关亚群 |
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| 作者单位： | 新疆医科大学基础医学院;新疆维吾尔自治区人民医院; |
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| 基金项目： | 新疆维吾尔自治区青年启动基金(2007Y17) |
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| 摘 要： | 目的运用序列特异性引物PCR技术（SSP-PCR）研究基因单核苷酸多态性。方法根据GenBank中人脂联素基因（APM1）序列及SNP信息,利用Primer5.0设计引物,通过PCR技术检测APM1的SNPs。结果建立并优化了序列特异性引物PCR（SSP-PCR）技术,并可快速、直观、准确地进行基因分型。结论改良的SSP-PCR技术是一种特异性更高、操作简便的SNP检测方法。
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| 关 键 词： | 序列特异性引物PCR 单核苷酸多态性 脂联素 |
Application of Improved SSP-PCR in Detection for Single Nucleotide Polymorphism |
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| WANG Yan-Jiao,SUN Li-li,CHEN Hong,JIAO Yi,LIU Xiao-yu,ZHANG Cheng,Akebar ALY,GUAN Ya-qun.Application of Improved SSP-PCR in Detection for Single Nucleotide Polymorphism[J].Endemic Diseases Bulletin,2010(2):4-6. |
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| Authors: | WANG Yan-Jiao SUN Li-li CHEN Hong JIAO Yi LIU Xiao-yu ZHANG Cheng Akebar ALY GUAN Ya-qun |
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| Institution: | WANG Yan-Jiao1,SUN Li-li1,CHEN Hong1,JIAO Yi1,LIU Xiao-yu1,ZHANG Cheng2,Akebar ALY2,GUAN Ya-qun1(1. Pre-clinical College of Xinjiang Medical University,Urumqi,Xinjiang 830054,China,2. People' Hospital of Xinjiang Uygur Autonomous Region,Xinjiang 830011,China) |
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| Abstract: | Objective To research single nucleotide polymorphism(SNP) of genes by sequence-specific-primers polymerase chain reaction(SSP-PCR). Methods According to information of adiponectin gene (APMI) of human from GenBank, specific primers were designed with Primer 5.0, and SNPs of APMI were detected by PCR. Results SSP-PCR was successfully established and improved. It could carry out genotyping rapidly, directly and exactly. Conclusions Improved SSP-PCR technique is a more specific and easier way to detect gene po... |
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| Keywords: | SSP-PCR Single nucleotide polymorphism Adiponectin |
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