乳腺癌患者外周血T淋巴细胞及NK细胞的检测及其临床意义

目的:探讨乳腺癌患者外周血T淋巴细胞亚群和NK细胞活性的临床意义。方法:采用流式细胞仪测定乳腺癌患者外周血T淋巴细胞亚群和NK细胞的活性,以正常人作对照。结果:乳腺癌患者外周血CD3 ,CD4 ,NK细胞数量及CD4 /CD8 比值较正常对照组均明显下降(P<0.05),而CD8 细胞水平显著升高。外周血T淋巴细胞亚群和NK细胞数量的改变与乳腺癌临床病理指标有关,分期越晚,CD3 细胞、CD4 细胞、CD4 /CD8 细胞比值及NK细胞数量越低,CD8 细胞水平越高;I、II期乳腺癌患者与III、IV期患者之间有显著差异(P<0.05)。结论:乳腺癌患者细胞免疫功能低下,且临床病理分期越晚,其免疫功能越低,检测T淋巴细胞亚群、NK细胞可用于乳腺癌患者的免疫监测。

Detection of T lymphocyte subset and NK cell in peripheral blood of the patients with breast cancer

Objective:To study the expression and clinical significance of T lymphocyte subset and NK cell activity in the patients with breast cancer . Methods: Flow cytometry was used to detect the marks of T lymphocyte subsets and NK cells in blood samples from patients with breast cancer. Health people was used as control. Results: Lower CD3~ , CD4~ T cells , NK cells and CD4~ /CD8~ ratio were detected in blood samples from cancer group than that from the health control (P<0.05) . CD8~ T lymphocyte was reverse . This change was related to the TNM pathological stage . Conclusion: The immune function of lymphocytes in peripheral blood of the patients with breast cancer was low , and even worse in the lowers grade of TNM stage . Removal of tumor may be benefit to improve the cellular immunity of cancer patients . It is valuable for estimating the function of cell immune by detecting T lymphocyte subset and NK cell.