恶性疟原虫对咯萘啶/磺胺多辛/乙胺嘧啶敏感性体外测定方法的研究

[目的 ]建立一种简便有效的体外微量测定方法 ,以监测恶性疟原虫对咯萘啶 /磺胺多辛 /乙胺嘧啶敏感性的变化。 [方法 ]设计制作咯萘啶 /磺胺多辛 /乙胺嘧啶 3种药物伍用测定板 ,采用WHO推荐的Rieckmann体外微量法 (WHO标准化法 )测定涂药板效果。测得的原始数据采用W H Wernsdorfer的专门软件进行分析 ,判定用体外微量法测定恶性疟原虫对上述 3种药物伍用测定板敏感性的可行性 ,并依据分数抑制浓度 (FIC)判定咯萘啶与磺胺多辛 /乙胺嘧啶之间是否有协同效应。 [结果 ]3药伍用测定板效果稳定 ,其ED5 0 分别为咯萘啶 0 11pmol/井、磺胺多辛 2 15 12pmol/井和乙胺嘧啶 2 6 9pmol/井。同时证明 ,咯萘啶与磺胺多辛 /乙胺嘧啶之间有协同效应。 [结论 ]恶性疟原虫对咯萘啶 /磺胺多辛 /乙胺嘧啶的敏感性可用体外微量法进行测定。

STUDIES ON THE SENSITIVITY OF PLASMODIUM FALCIPARUM TO PYRONARIDINE /SULFADOXINE/PYRIMETHAMINE IN VITRO

Objective] To develop an in vitro method for the assessment of drug response in P falciparum to pyronaridine/sulfadoxine/pyrimethamine(PND/S/P).[Methods]The PND/S/P microtest plate was designed,Rieckmann in vitro microtest(WHO standard kit) was used to test the sensitivity of P falciparum in continuous culture(FCC1/HN strain), and the data obtained were analyzed using a computer programme. Fractional inhibitory concentration (FIC) was calculated to test the possible synergy between PND and S/P. [Results]:The effect of the PND/S/P plates was fairly stable and the ED 50 values of pyronaridine,sulfadoxine and pyrimethamine were 0 11,215 12 and 2 9 pmol,respectively. The FIC obtained confirmed the synergism between PND and S/P. [Conclusion]The in vitro method can be used to assess the sensitivity of P. falciparum to pyronaridine /sulfadoxine/pyrimethamine.