| HPLC法测定复方抗病毒口服液中绿原酸的含量 |
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| 引用本文： | 唐德智.HPLC法测定复方抗病毒口服液中绿原酸的含量[J].中国中医药信息杂志,2010,17(1):42-43. |
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| 作者姓名： | 唐德智 |
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| 作者单位： | 南宁食品药品检验所,广西,南宁,530001 |
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| 摘 要： | 目的建立HPLC法测定复方抗病毒口服液中绿原酸含量的方法。方法采用Shim-pack VP-ODS C18反相色谱柱（4．6mm×250mm，5μm），以乙腈-0．2％磷酸（12：88）为流动相；流速为1．0mL／min；检测波长：327nm；柱温：30℃。结果绿原酸在0．051～0．922Pg范围内线性关系良好（r=0．9999，n=6），平均回收率为99．04％，RSD=1．31％（n=6）。结论该方法准确、重复性好、简便易行，可作为复方抗病毒口服液的质量控制方法。
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| 关 键 词： | 复方抗病毒口服液 绿原酸 高效液相色谱法 含量测定 |
Determination of Chlorogenic Acid in Compound Oral Liquid of Kangbingdu by HPLC |
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| TANG De-zhi.Determination of Chlorogenic Acid in Compound Oral Liquid of Kangbingdu by HPLC[J].Chinese Journal of Information on Traditional Chinese Medicine,2010,17(1):42-43. |
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| Authors: | TANG De-zhi |
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| Institution: | TANG De-zhi(Nanning Institute for Food , Drug Control,Nanning 530001,China) |
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| Abstract: | Objective To establish a method for detecting chlorogenic acid in compound oral liquid of Kangbingdu with HPLC. Methods The chromatographic column of Shim-pack VP-ODS C18 （4.6 minx 250 mm, 5 μm） was used, the mobile phase was consisted of acetonitrile-0.2% phosphoric acid aqueous solution （12 ： 88）. The flow rate was 1.0 mL/min, the detective wavelength was 327 nm and column temperature was 30 ℃. Results The linear range of the method was 0.051-0.922 μg （r =0.999 9, n=6）, the average recovery was 99.04%, RSD was 1.31% （n=6）. Conclusion The method is accurate, reproducible, simple and easy to do, which can be used in quality control of compound oral liquid of Kangbingdu. |
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| Keywords: | compound oral liquid of Kangbingdu chlorogenic acid HPLC content determination |
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