PCNA和Ki-67在染锰大鼠生精细胞中的表达及研究

目的研究氯化锰对生精细胞PCNA和Ki-67表达的影响，比较二者作为细胞增殖指标的差异。方法雄性sD大鼠（体重120±10g）48只，随机分为6组：空白对照组，低剂量（15mg／kgMnCl2）和高剂量（30mg／kgMnCl2）组，8只／组。MnCl2组分别染锰4周和6周，空白对照组给予等量NS，给药途径均为腹腔注射，5d／周，1次／d，分别于第4周末和第6周末处死，免疫组化（SABC法）法检测睾丸PCNA和Ki-67表达。结果与空白对照组比较，染锰4周PCNA阳性细胞率显著降低，染锰6周反而升高（P〈0．01）。各染锰组Ki-67总阳性细胞率显著降低，细胞核阳性细胞率显著升高（P〈0．01）。染锰剂量相同，6周与4周组比较，PCNA阳性细胞率显著升高，Ki-67总阳性细胞率显著降低，Ki-67细胞核阳性细胞率显著升高（P〈0．01）。染锰时间相同，高剂量组与低剂量组比较，PCNA阳性细胞率和Ki-67总阳性细胞率均显著降低（P〈0．01），Ki-67细胞核阳性细胞率在4周组和6周组分别显著升高和显著降低（P〈0．01）。结论15mg／kg氯化锰即可抑制大鼠生精细胞的增殖能力，PCNA和Ki-67互相结合可以更加准确地反映细胞周期。

Comparative Study on the Expression of PCNA and Ki- 67 in Spermatogenic Cells of Rats Exposed to Manganese

Objective Our purpose was to study the the expression of PCNA and Ki - 67in spermat- ogenic cells of male rats exposed to manganese, and to discuss the difference of them as indexes of proliferative function of eel1. Methods 48 male rats （weighting 120 ±0 g） were randomly divided into 6 groups （blank control, 15 mg/kg MnCl2, 30 mg/kg MnCl2） , There were 8 rats in every group. 15 mg/ kg MnCl2 or 30 mg/kg MnCl2 groups were contaminated manganese （MnCl2 ~ 4H20）by I. P. for 4 and 6 weeks respectively and blank control group was injected with NS. All rats were injected once everyday, five times per week. In the 4th and 6th weekend, their testis were collected. The expression of PCNA and Ki -67 were investigated by SABC. Results Compared with blank control group, the expression of PCNA and Ki - 67 decreased in 4 w manganese exposure group, but increased in 6 w group （ P 〈 0.01 ） , the totle rate of Ki - 67 - positive - cell decreased but the Ki - 67 - nucleus - positive - cell rate in- creased in every manganese exposure groups （P 〈 0.01 ）. Among manganese exposure groups of same dose, compared with those in 4 w exposure group, the PCNA - positive - cell rate increased and the total rate of Ki - 67 - positive - cell decreased but the Ki - 67 - nucleus - positive - cell rate increased in 6w group （P 〈0.01 ）. Among manganese exposure groups of same time, compared with those in 15 mg/kg group, the PCNA - positive - cell rate and the total rate of Ki - 67 - positive - cell decreased, but the Ki - 67 - nucleus - positive - cell rate increased or decreased respectively in 4 w and 6 w group in 30 rag/ kg groups. Conclusion MnCl2 in dose of 15 mg/kg can inhibit proliferative function of spermatogenic cells in rats, the combination of PCNA and Ki -67 can reflected proliferative function of cells more accu- rately.