幽门螺旋杆菌不同检测方法的比较

目的：通过对不同的幽门螺旋杆菌检测方法灵敏性和特异性进行比较，为临床提供一种检测幽门螺旋杆菌的最佳方法。方法：在距幽门5cm区域取活组织并抽取静脉血2ml，采用Gram染色法、快速尿素酶试验法、ELISA法和PCR法分别检测幽门螺旋杆菌。结果：Gram染色法、快速尿酶试验法、ELISA法和PCR法对幽门螺旋杆菌的检测灵敏性分别为83．6％、88．4％、92．9％、97．3％，特异性分别为78．3％、77．0％、85．0％、87．5％。结论：4种方法中以PCR检测法的灵敏性和特异性最高，抗体检测法次之。而检测结果与实际情况总符合率除Gram染色法稍低外，其它3种方法之间无明显差异。如果能采用两种检测手段同时检测，灵敏性则几乎可达到100％。

The Comparison of Different Methods for the Detection of H. Pylori

Objective:It was reported that H. Pylori is one of the high risk factors in chronic gastritis, ulcer, and gastric cancer. The aim of this study is to provide a useful method for detecting H. Pylori. Methods: 90 patients were detected H. Pylori with histology, urease testing, ELISA technique, and PCR technique. Rusults: The sensitivity of four methods is: histology (83.6%), urease testing (88.4%),ELISA technique (92.9%), and PCR technique (97.3%). The differential of four methods is histology (78.3%), urease testing (77.0%), ELISA technique (85.0%), and PCR technique (87.5%). Conclusion: PCR technique is the most sensitivity and differential method.If two methods are used at the same time, the sensitivity of detection of H. Pylori was almost achieved 100%.