MOUSE AND HUMAN MONOCLONAL ANTIBODIES AGAINSTGASTRIC CANCERPREPARATION AND CLINICAL APPLICATION

Ten clones of mouse and two clones, oF hu-man phybridoma cell producing monoclonal an- tibodies against gastric cancer were established. The nature, specificity and affinity of these mo- noclonal antibodies for gastric cancer associated antigens were demonstrated. They were used preliminarily in the diagnosis of gastric carcino- ma with encouraging results. Cultivated gastric cancer cells or tumor cells from surgically resected gastric cancer or affected lymph nodes were used to immunize Balb/c mice. The lymphocytes harvested from the spleen after immunization were fused with mouse myeloma cell line SP2/0. Ten kinds of mouse monoclonal antibodies reactive with different histopathologi- cal types of gastric carcinomas have been prepar~ ed. Two kinds of human monoclonal antibodies have also been produced by fusion of the lym- phocytes from the draining lymph nodes with mouse-human heteromyeloma cell line SHM-D33. Considerable amounts of McAbs can be obtained from the cell culture supernatant or from ascitic fluid of mice with intraperitoneally transplanted hybridoma cells.