供体特异性输血诱导CD8+CD28-调节性T细胞在肝移植急性排斥反应中的作用

目的 通过供体特异性输血(DST)诱导机体产生抗原特异性CD8+CD28-调节性T细胞(CD8+CD28-Tr),评价其对大鼠肝移植急性排斥反应的抑制作用.方法 采用DST在正常BrownNorway(BN)大鼠体内诱导针对LEW抗原的CD8+CD28-Tr,体外验证其免疫抑制活性之后将其输注给LEW→BN肝移植急性排斥模型受体,观察输注后大鼠的生存时间和肝脏病理学表现.采用SPSS11.0软件进行Log-Rank检验,比较大鼠生存率的差异.结果 DST可在正常BN大鼠体内诱导大量CD8+CD28-Tr产生,其在体外具有免疫抑制活性,体内输注可缓解大鼠肝移植急性排斥反应(LEw→BN).结论 DST可以在正常大鼠体内诱导大量CD8+CD28-Tr产生,其体内输注可以抑制大鼠肝移植急性排斥反应,DST可能有望成为体内诱导特异性CD8+CD28-Tr的途径之一.

The induction of CD8+CD28- regulatory T cells by donor specific transfusion and their effect on the acute rejection responses in rats liver transplantation

Objective To acquire antigen specific CD8+CD28-Tr by donor specific transfusion (DST) in vivo, and evaluate their suppressive effect on the acute rejection responses in rat liver transplantation through adoptive transfer experiment. Methods DST was used to induce CD8 + CD28 Tr to LEW antigens in naive Brown Norway(BN) rat. Then the induced CD8+CD28- Tr were adoptively transferred into the recipients of LEW→BN liver transplantation, which were acute rejection models. The survival time and histological changes were observed. Statistic analysis was performed with Log-Rank test by SPSS11.0 software, to compare the survival rate. Results Adoptive transfer of the DST-induced CD8+CD28-Tr attenuated the acute rejection responses in acute rejection models of rat liver transplantation. Conclusion DST can induce large numbers of CD8+CD28-Tr in naive BN rats in vivo. The adoptive transfer of the induced CD8+CD28Tr suppressed the acute rejection responses in rat liver transplantation. DST may become one of the methods that induce antigen specific CD8+CD28- Tr in vivo.